Epilepsy Antibody Evaluation with Reflex to Titer and Line Blot, Serum
Test Code
94956
86255 (x19), 86052, 86341, 83519 (x2), 86596 (x2)
In-home collection is not available in your area through Quest Mobile.
Clinical Significance
Epilepsy Antibody Evaluation with Reflex to Titer and Line Blot, Serum - The Epilepsy Antibody Evaluation panel provides an evaluation of possible autoantibodies in patients with suspected autoimmune epilepsy, and optimizes the likelihood of detecting neuronal specific autoantibodies, whether present singly or occurring as multiple autoantibodies.
Test Details
Initial panel consists of: Epilepsy Ab Screen Tissue IFA, AMPAR1, AMPAR2, Aquaporin 4, DPPX, GABAbR, NMDAR1, CASPAR2, LGI1, Ganglionic AChR, VGCC N-Type, VGCC P/Q-Type, and VGKC.
1) A line blot consisting of 10 analytes will be performed at an additional charge (CPT code(s): 84182 x10) as a reflex for tissue mosaic IFA suggesting one or more of the analytes on the line blot: ANNA1 (Hu), ANNA2 (Ri), PCA1 (Yo), Ma2/Ta, CV2 (CRMP5), Amphiphysin, AGNA1 (SOX1), GAD65, DNER, and Zic4. If the tissue mosaic pattern suggests ANNA3, then titer will be performed at an additional charge (CPT code(s): 86256).
2) If the tissue mosaic pattern suggests PCA-2, then titer will be performed at an additional charge (CPT code(s): 86256).
3) If the tissue mosaic IFA suggests PCA-Tr (DNER) and Western Blot shows DNER negative and Yo negative, then cell based assay IFA for DNER will be performed at an additional charge (CPT code(s): 86255).
a. If DNER CBA is positive, then titer will be performed at an additional charge (CPT code(s): 86256).
4) If the tissue mosaic IFA suggests myelin antibody, then
a. Myelin antibody IFA titer will be performed at an additional charge (CPT code(s): 86256).
b. Myelin Associated Glycoprotein (MAG) Antibody, in turn reflexing to MAG-SGPG and MAG ELISA for quantitation, will be performed at an additional charge (CPT code(s): 83520 x2).
5) If the Mosaic CBA is positive for any given analyte (NMDAR1, AMPAR1, AMPAR2, GABA-B Receptor, LGI-1, CASPR2), and the individual CBAs DPPX and Aquaporin 4 antibody, then that analyte will be titered at an additional charge (CPT code(s): 86256 for each titer performed, 86052 for AQP4 Titer).
6) If DPPX is positive, then titer will be performed at an additional charge (CPT code(s): 86256).
7) If the Aquaporin 4 (NMO, neuromyelitis optica) CBA is positive, then Aquaporin 4 CBA titer will be performed at an additional charge (CPT code(s): 86052).
1) A line blot consisting of 10 analytes will be performed at an additional charge (CPT code(s): 84182 x10) as a reflex for tissue mosaic IFA suggesting one or more of the analytes on the line blot: ANNA1 (Hu), ANNA2 (Ri), PCA1 (Yo), Ma2/Ta, CV2 (CRMP5), Amphiphysin, AGNA1 (SOX1), GAD65, DNER, and Zic4. If the tissue mosaic pattern suggests ANNA3, then titer will be performed at an additional charge (CPT code(s): 86256).
2) If the tissue mosaic pattern suggests PCA-2, then titer will be performed at an additional charge (CPT code(s): 86256).
3) If the tissue mosaic IFA suggests PCA-Tr (DNER) and Western Blot shows DNER negative and Yo negative, then cell based assay IFA for DNER will be performed at an additional charge (CPT code(s): 86255).
a. If DNER CBA is positive, then titer will be performed at an additional charge (CPT code(s): 86256).
4) If the tissue mosaic IFA suggests myelin antibody, then
a. Myelin antibody IFA titer will be performed at an additional charge (CPT code(s): 86256).
b. Myelin Associated Glycoprotein (MAG) Antibody, in turn reflexing to MAG-SGPG and MAG ELISA for quantitation, will be performed at an additional charge (CPT code(s): 83520 x2).
5) If the Mosaic CBA is positive for any given analyte (NMDAR1, AMPAR1, AMPAR2, GABA-B Receptor, LGI-1, CASPR2), and the individual CBAs DPPX and Aquaporin 4 antibody, then that analyte will be titered at an additional charge (CPT code(s): 86256 for each titer performed, 86052 for AQP4 Titer).
6) If DPPX is positive, then titer will be performed at an additional charge (CPT code(s): 86256).
7) If the Aquaporin 4 (NMO, neuromyelitis optica) CBA is positive, then Aquaporin 4 CBA titer will be performed at an additional charge (CPT code(s): 86052).
Reference Range(s)
See Laboratory Report
Alternative Name(s)
Autoimmune Epilepsy Panel,Epilepsy-Autoimmune Evaluation
Preferred Specimen(s)
8 mL frozen serum
Minimum Volume
4 mL
Transport Container
Transport tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: 24 hours
Refrigerated: 14 days
Frozen: 21 days
Reject Criteria
Gross hemolysis • Grossly lipemic • Grossly icteric • Freeze/thaw greater than 3 cycles • Turbid • Bacterial contamination
Setup Schedule
8 mL frozen serum
4 mL
Transport tube
Refrigerated (cold packs)
Room temperature: 24 hours
Refrigerated: 14 days
Frozen: 21 days
Refrigerated: 14 days
Frozen: 21 days
Gross hemolysis • Grossly lipemic • Grossly icteric • Freeze/thaw greater than 3 cycles • Turbid • Bacterial contamination