Hepatitis C Antibody with Reflex to HCV RNA, PCR with Reflex to Genotype

Hepatitis C Antibody with Reflex to HCV RNA, PCR with Reflex to Genotype

Test Code

94345
86803
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
94345
86803
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Hepatitis C Antibody with Reflex to HCV RNA, PCR with Reflex to Genotype - Hepatitis C Virus (HCV) is the major cause of hepatitis. Making positive HCV Ab tests reflex to confirmatory molecular testing is necessary to confirm active HCV infection. Providing a second reflex to perform genotyping on HCV isolates when RNA is detected is also a necessary step before starting therapy. This double reflex will reduce the percentage of antibody-positive patients who lack appropriate confirmatory RNA testing and who do not have genotype testing performed.

Test Resources

Test FAQ

Hepatitis C Antibody with Reflex to HCV RNA, PCR with Reflex to Genotype

Clinical Focus

Viral Hepatitis: Laboratory Support for Diagnosis and Management

Test Guide

HIV Infection: Laboratory Testing for Diagnosis and Management

Test Details

Includes

  • If Hepatitis C Antibody is reactive or borderline, then Hepatitis C Viral RNA, Quantitative, Real-Time PCR will be performed at an additional charge (CPT code(s): 87522).
    If Hepatitis C Viral RNA, Quantitative, Real-Time PCR is ≥2000 IU/mL, then Hepatitis C Virus RNA Genotype will be performed at an additional charge (CPT code(s): 87902).

Methodology

Immunoassay (IA)

Reference Range(s)

Non-Reactive

Alternative Name(s)

HCV RNA Genotype,Hep C Genotype,HEP C Virus Genotype,HEP C RNA QNT PCR W/RFX Genotype,Hepatitis C RNA Genotype,HCV with Reflex,HCV Antibody

LOINC® Codes, Performing Laboratory

Service Area must be determined
If Hepatitis C Antibody is reactive or borderline, then Hepatitis C Viral RNA, Quantitative, Real-Time PCR will be performed at an additional charge (CPT code(s): 87522).
If Hepatitis C Viral RNA, Quantitative, Real-Time PCR is ≥2000 IU/mL, then Hepatitis C Virus RNA Genotype will be performed at an additional charge (CPT code(s): 87902).

Methodology

Immunoassay (IA)

Reference Range(s)

Non-Reactive

Alternative Name(s)

HCV RNA Genotype,Hep C Genotype,HEP C Virus Genotype,HEP C RNA QNT PCR W/RFX Genotype,Hepatitis C RNA Genotype,HCV with Reflex,HCV Antibody
Service Area must be determined

Preferred Specimen(s)

2.5 mL serum collected in each of 2 serum separator tubes (SST)

Alternative Specimen(s)

Plasma collected in: Each of 2 EDTA (lavender-top) tubes

Minimum Volume

2 mL (x2)

Transport Container

Serum separator tube (SST)

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 72 hours
  • Refrigerated: 14 days
  • Frozen: 30 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Red-top tube (no gel) • Heparinized plasma

Setup Schedule

Service Area must be determined
2.5 mL serum collected in each of 2 serum separator tubes (SST)
Plasma collected in: Each of 2 EDTA (lavender-top) tubes
2 mL (x2)
Serum separator tube (SST)
Room temperature
Room temperature: 72 hours
Refrigerated: 14 days
Frozen: 30 days
Gross hemolysis • Grossly lipemic • Red-top tube (no gel) • Heparinized plasma
Service Area must be determined
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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