QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)

QuestAssureD™ 25-Hydroxyvitamin D (D2, D3)

Test Code

92888
82306
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
92888
82306
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

QuestAssureD™ 25-Hydroxyvitamin D (D2, D3) - Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25-OH ...

Test Resources

Test FAQ

Vitamin D Testing

Test Details

Includes

  • Vitamin D, 25-OH, Total
  • Vitamin D, 25-OH, D3
  • Vitamin D, 25-OH, D2

Patient Preparation

Fasting preferred, but not required

Methodology

Chromatography/Mass Spectrometry

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established

Alternative Name(s)

Vitamin D3, 25-hydroxy,D2 Vitamin,D3 Vitamin,25-Hydroxycholecalciferol (25OHD3),25-Hydroxyergocalciferol (25OHD2),25-Hydroxyvitamin D2,25-Hydroxyvitamin D3,25-OH-D2,25-OH-D3,Vit D, LC/MS/MS,Vitamin D2, 25-hydroxy

LOINC® Codes, Performing Laboratory

Service Area must be determined
Vitamin D, 25-OH, Total
Vitamin D, 25-OH, D3
Vitamin D, 25-OH, D2
Fasting preferred, but not required

Methodology

Chromatography/Mass Spectrometry
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Vitamin D, 25-OH, Total 30-100 ng/mL
Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established

Alternative Name(s)

Vitamin D3, 25-hydroxy,D2 Vitamin,D3 Vitamin,25-Hydroxycholecalciferol (25OHD3),25-Hydroxyergocalciferol (25OHD2),25-Hydroxyvitamin D2,25-Hydroxyvitamin D3,25-OH-D2,25-OH-D3,Vit D, LC/MS/MS,Vitamin D2, 25-hydroxy
Service Area must be determined

Preferred Specimen(s)

0.5 mL serum

Minimum Volume

0.3 mL

Collection Instructions

Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 21 days
  • Refrigerated: 21 days
  • Frozen: 21 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from serum separator tube (SST) gel or clot within 48 hours • Grossly icteric

Setup Schedule

Service Area must be determined
0.5 mL serum
0.3 mL
Collect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Transport tube
Room temperature
Room temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days
Gross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from serum separator tube (SST) gel or clot within 48 hours • Grossly icteric
Service Area must be determined
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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