Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation
Test Code
Clinical Significance
Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation - Measurement of human lymphocytes' proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Mitogens, such as plant lectins phytohemagglutinin (PHA), concanavalin A (Con A) and pokeweed mitogen (PWM), are able to nonspecifically stimulate lymphocyte proliferation and used to evaluate patient immune responsiveness.
Test Resources
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Test Details
Methodology
Reference Range(s)
| Males | 73700-265000 Net CPM |
| Females | 53000-200000 Net CPM |
| PHA, SI | Not established |
| Cell Viability | 80-100% |
Alternative Name(s)
Preferred Specimen(s)
5 mL whole blood collected in a sodium heparin (green-top) tube
Minimum Volume
3 mL
Collection Instructions
Whole blood must be transported at room temperature and delivered to the testing laboratory within 48 hours after collection.
Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37° C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday-Thursday only. Do not draw day before a holiday. Samples received in testing lab >48 hours after collection will be rejected. Date and time of draw are required and must be provided.
Transport Container
Sodium heparin (green-top) tube
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 48 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable
Reject Criteria
Hemolysis • Received refrigerated • Received frozen • Clotted specimens • Samples collected in lithium heparin tubes
Setup Schedule
Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37° C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation.
Clients: Contact the lab prior to ordering for special logistics arrangements.
PSC: Follow Short Stability Protocol.
Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions.
Submit Monday-Thursday only. Do not draw day before a holiday. Samples received in testing lab >48 hours after collection will be rejected. Date and time of draw are required and must be provided.
Refrigerated: Unacceptable
Frozen: Unacceptable