Phytohemagglutinin (PHA)-induced Lymphocyte Proliferation
Test code
CPT code(s)*
Test resources
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Details
Methodology
Cell Culture (CC) • Scintillation Counter
Assay category
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Alternative name(s)
Mitogen-induced Cell Proliferation,Lymphocyte Stimulation
Specimen requirements | |
|---|---|
| Patient preparation | None Specified |
| Preferred specimen(s) | 5 mL whole blood collected in a sodium heparin (green-top) tube |
| Minimum volume | 3 mL |
| Collection instructions | Whole blood must be transported at room temperature and delivered to the testing laboratory within 48 hours after collection. Maintain and transport blood at room temperature. Avoid temperatures <15° C and >37° C. In hot weather, it may be necessary to pack the specimen in a container with insulating material around it and place this container inside another one that contains a cold pack (ice pack) and absorbent material. This method will help retain the specimen at ambient temperature. For longitudinal studies, draw specimens at the same time of day to minimize diurnal variation. Clients: Contact the lab prior to ordering for special logistics arrangements. PSC: Follow Short Stability Protocol. Test available by prior arrangement only. Special sample collection and transportation arrangements must be made prior to ordering the test. Contact your local Customer Service Department and request to speak to someone in Referral Testing Department for specific instructions. Submit Monday-Thursday only. Do not draw day before a holiday. Samples received in testing lab >48 hours after collection will be rejected. Date and time of draw are required and must be provided. |
| Transport container | Sodium heparin (green-top) tube |
| Transport temperature | Room temperature |
| Specimen stability | Room temperature: 48 hours Refrigerated: Unacceptable Frozen: Unacceptable |
| Reject criteria | Hemolysis • Received refrigerated • Received frozen • Clotted specimens • Samples collected in lithium heparin tubes |
Reference Range(s)
PHA, CPM
| Males | 73700-265000 Net CPM |
| Females | 53000-200000 Net CPM |
| PHA, SI | Not established |
| Cell Viability | 80-100% |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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