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Cardio IQ® Vitamin D, 25-Hydroxy

Test code

91735

CPT code(s)*

82306
Notices
**CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide Service Area Information to find available tests you can order.
In-home collection may be available in your area for purchase through  Quest Mobile.

Clinical significance

Cardio IQ® Vitamin D, 25-Hydroxy - Measurement of serum 25-OH vitamin D concentrations provide a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead hypercalcemia. This assay employs liquid chromatography tandem mass spectrometry to independently measure and report the two common forms of 25-hydroxy vitamin D: 25OH D3 - the endogenous form of the…
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Test resources

None found for this test
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Details

Test details

Includes

Vitamin D, 25-OH, Total
Vitamin D, 25-OH, D3
⁠⁠⁠⁠⁠⁠⁠Vitamin D, 25-OH, D2

Methodology

Chromatography/Mass Spectrometry

Assay category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Alternative name(s)

CardioIQ®,Vitamin D, 25-Hydroxy, LC/MS/MS, Cardio IQ®

Specimen requirements
Patient preparationFasting preferred, but not required
Preferred specimen(s)0.5 mL serum
Minimum volume0.3 mL
Collection instructionsCollect blood in a standard red-top serum Vacutainer® tube. Allow blood to clot at room temperature. Centrifuge and separate the serum from the cells immediately.
Alternatively, collect blood in a Serum separator tube (SST), allow to clot at room temperature. Centrifuge and remove from the gel within 48 hours.
Transport containerTransport tube
Transport temperatureRefrigerated (cold packs)
Specimen stabilityRoom temperature: 21 days
Refrigerated: 21 days
Frozen: 21 days
Reject criteriaGross hemolysis • Grossly lipemic • Heparinized or EDTA plasma • Serum not separated from Serum separator tube (SST) gel or clot within 48 hours • Grossly icteric

Vitamin D, 25-OH, Total 30-100 ng/mL
25-OHD3 indicates both endogenous production and supplementation. 25-OHD2 is an indicator of exogenous sources, such as diet or supplementation. Therapy is based on measurement of Total 25-OHD, with levels <20 ng/mL indicative of Vitamin D deficiency, while levels between 20 ng/mL and 30 ng/mL suggest insufficiency. Optimal levels are ≥30 ng/mL.

Vitamin D, 25-OH, D3 Not established
Vitamin D, 25-OH, D2 Not established

Service Area Information
Service Area Information

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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