HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)

HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase)

Test Code

91691
87536
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
91691
87536
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
In-home collection is not available in your area through Quest Mobile.

Clinical Significance

HIV-1 RNA, Quantitative, Real-Time PCR with Reflex to Genotype (RTI, PI, Integrase) - Monitor viral load in HIV-1 patients undergoing antiretroviral therapy and automatically reflex to drug resistance testing for PR, RT and Integrase drugs in patients failing to achieve or maintain virologic suppression.

Test Resources

Test FAQ

HIV-1 Genotype

Test FAQ

HIV-1 Integrase Genotype

Test Guide

HIV Infection: Laboratory Testing for Diagnosis and Management

Test Details

Includes

  • If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, then HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge (CPT code(s): 87900, 87901, 87906).

Methodology

Real-Time Polymerase Chain Reaction

Reference Range(s)

HIV-1 RNA, QN, PCRNot detectedcopies/mL
HIV-1 RNA, QN, PCRNot detectedLog copies/mL

Alternative Name(s)

Human Immunodeficiency Virus

LOINC® Codes, Performing Laboratory

Service Area must be determined
If HIV-1 RNA, Quantitative, Real-Time PCR viral load is >400 copies/mL, then HIV-1 Genotype and HIV-1 Integrase Genotype will be performed at an additional charge (CPT code(s): 87900, 87901, 87906).

Methodology

Real-Time Polymerase Chain Reaction

Reference Range(s)

HIV-1 RNA, QN, PCRNot detectedcopies/mL
HIV-1 RNA, QN, PCRNot detectedLog copies/mL

Alternative Name(s)

Human Immunodeficiency Virus
Service Area must be determined

Preferred Specimen(s)

7 mL plasma collected in an EDTA (lavender-top) tube

Alternative Specimen(s)

Plasma collected in: PPT Potassium EDTA (white-top) tube

Minimum Volume

3.7 mL

Collection Instructions

Samples submitted in primary tube will be rejected. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.Follow manufacturer's instructions for collection tube handling.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

  • Room temperature: Unacceptable
  • Refrigerated: 6 days
  • Frozen: 30 days

Reject Criteria

Gross hemolysis • Lipemia • Samples received in primary collection tube

Setup Schedule

Service Area must be determined
7 mL plasma collected in an EDTA (lavender-top) tube
Plasma collected in: PPT Potassium EDTA (white-top) tube
3.7 mL
Samples submitted in primary tube will be rejected. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube.Follow manufacturer's instructions for collection tube handling.
Transport tube
Frozen
Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 30 days
Gross hemolysis • Lipemia • Samples received in primary collection tube
Service Area must be determined
In-home collection is not available in your area through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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