HIV-1/2 Antigen and Antibodies, Fourth Generation, with Reflexes
Test Code
Clinical Significance
HIV-1/2 Antigen and Antibodies, Fourth Generation, with Reflexes - This algorithm is used to help diagnose HIV-1 and HIV-2 infection, including acute infection, and to differentiate HIV-1 from HIV-2. It is consistent with the HIV diagnostic algorithm recommended by the Centers for Disease Control and Prevention [2]. It can be used in adults, including pregnant women, and in children 2 years or older.
This panel begins with an antigen/antibody assay that allows for detection of acute HIV-1 infection based on the presence of p24 antigen before seroconversion occurs, and HIV-1 and HIV-2 antibodies post-seroconversion. If the HIV-1/HIV-2 antigen/antibody test ...
Test Resources
Test Details
If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 and HIV-2 RNA, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87535, 87538).
Methodology
Reference Range(s)
| HIV Final Interpretation | See Laboratory Report |
| HIV Ag/Ab, 4th Gen | Non-Reactive |
Alternative Name(s)
Preferred Specimen(s)
3 mL serum
Alternative Specimen(s)
Serum collected in: Red-top tube (no gel) • Plasma collected in: EDTA (lavender-top) tube
Minimum Volume
2 mL
Collection Instructions
Serum: Collect in a serum separator tube or red-top tube (no gel). The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic screw-cap vial. Red-top tube (no gel) serum should be transferred to a plastic screw-cap vial and submitted for testing.
Plasma: Collect in an EDTA (lavender-top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma, and submit for testing.
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Transport Container
Serum separator tube (SST)
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 72 hours
- Refrigerated: 5 days
- Frozen: 30 days
Reject Criteria
Gross hemolysis • Frozen serum separator tube (SST)
Setup Schedule
Plasma: Collect in an EDTA (lavender-top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma, and submit for testing.
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Refrigerated: 5 days
Frozen: 30 days