HIV-1/2 Antigen and Antibodies, Fourth Generation, with Reflexes
Test code
CPT code(s)*
Clinical significance
Test resources
Algorithm
Clinical focus
Details
Includes
If HIV Antigen and Antibody, 4th Generation Screen is Reactive, then HIV-1/2 Antibody Differentiation will be performed at an additional charge (CPT code(s): 86701, 86702).
If HIV-1/2 Antibody Differentiation is Indeterminate or Negative, then HIV-1 and HIV-2 RNA, Qualitative Real-Time PCR will be performed at an additional charge (CPT code(s): 87535, 87538).
Methodology
Immunoassay (IA)
Alternative name(s)
Fourth Generation HIV,4th Generation HIV
Specimen requirements | |
|---|---|
| Patient preparation | None Specified |
| Preferred specimen(s) | 3 mL serum |
| Alternative specimen(s) | Serum collected in: Red-top tube (no gel) • Plasma collected in: EDTA (lavender-top) tube |
| Minimum volume | 2 mL |
| Collection instructions | Serum: Collect in a serum separator tube or red-top tube (no gel). The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic screw-cap vial. Red-top tube (no gel) serum should be transferred to a plastic screw-cap vial and submitted for testing. Plasma: Collect in an EDTA (lavender-top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma, and submit for testing. Submitted tubes must be labeled with patient identifier and submitted only for HIV testing. |
| Transport container | Serum separator tube (SST) Submitted tubes must be labeled with patient identifier and submitted only for HIV testing. |
| Transport temperature | Room temperature |
| Specimen stability | Room temperature: 72 hours Refrigerated: 5 days Frozen: 30 days |
| Reject criteria | Gross hemolysis • Frozen serum separator tube (SST) |
Reference Range(s)
| HIV Final Interpretation | See Laboratory Report |
| HIV Ag/Ab, 4th Gen | Non-Reactive |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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