HIV-1 Coreceptor Tropism, Proviral DNA
Test code
CPT code(s)*
Test resources
Details
Includes
CXCR4(X4)
Net Tropism Assessment
MVC Activity Anticipated
Methodology
DNA Sequencing • Polymerase Chain Reaction (PCR)
Assay category
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Alternative name(s)
Trofile™,CCR5,CXCR4,Human Immunodeficiency Virus,R5,X4
Specimen requirements | |
|---|---|
| Patient preparation | None Specified |
| Preferred specimen(s) | 2 mL whole blood collected in a K2 EDTA (lavender-top) plastic tube |
| Alternative specimen(s) | 3 mL peripheral blood mononuclear cells |
| Minimum volume | 0.6 mL |
| Collection instructions | Whole blood: Collect blood in a K2 EDTA (lavender-top) tube. Peripheral blood mononuclear cells: Collect blood in BD Vacutainer® CPT™ tube. After collection, store tube upright at room temperature for up to two hours before centrifugation. Remix blood sample immediately prior to centrifugation by inverting 10 times. Centrifuge at 800g for 30 min, room temperature with brake off. Immediately remove plasma (≥3 mL) from top layer without disturbing cell layer. Add 0.5 mL phosphate-buffered saline to remaining layer of plasma/cells. Cap tube and gently mix by inversion 10 times. Transfer the upper layer of cells (up to 3 mL) in plasma/phosphate-buffered saline to a sterile K2 EDTA tube. Store and ship frozen at <-60° C. Do not send original glass CPT™ or plasma supernatant to the lab. |
| Transport container | K2 EDTA (lavender-top) plastic tube |
| Transport temperature | Whole blood: Room temperature Peripheral blood: Frozen |
| Specimen stability | Whole blood Room temperature: 7 days Refrigerated: 7 days Frozen: 28 days Peripheral blood mononuclear cells Room temperature: See Collection Instructions Refrigerated: See Collection Instructions Frozen -20° C: See Collection Instructions Frozen -70° C: 28 days |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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