BCR-ABL1 Gene Rearrangement, Quantitative, PCR
Test code
CPT code(s)*
If P210 transcript expression was previously documented, only P210 BCR-ABL1 will be performed (CPT code(s): 81206).
If no prior positive is documented, P190 BCR-ABL1 and P210 BCR-ABL1 will be performed (CPT code(s): 81206, 81207).
CPT coding may differ dependent on payer rules which may impact prior authorization testing.
Please direct any questions regarding CPT coding to the payer being billed.
Clinical significance
Details
Methodology
Quantitative Real-Time Polymerase Chain Reaction (PCR)
Assay category
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Alternative name(s)
P190 BCR-ABL transcript,P210 BCR-ABL transcript,t(9;22),Philadelphia Chromosome
Specimen requirements | |
|---|---|
| Patient preparation | None Specified |
| Preferred specimen(s) | 4 mL whole blood or 3 mL bone marrow collected in an EDTA (lavender-top) tube |
| Alternative specimen(s) | Whole blood or bone marrow collected in: Sodium heparin (green-top) tube • 50 uL of 10 ng/uL extracted RNA submitted in a microcentrifuge tube |
| Minimum volume | 3 mL whole blood • 1 mL bone marrow • 20 uL extracted RNA |
| Collection instructions | Do not reject specimens, send to laboratory for screening. Collect 4 mL whole blood or 3 mL bone marrow in an EDTA (lavender-top) tube. Whole blood or bone marrow is shipped at room temperature or refrigerated (cold packs). Do not freeze whole blood or bone marrow. After collection of the sample, draw date and time, as well as sample type, must be written on the tube and included as requested information. If the stability of the sample cannot be determined, delay in result or cancellation of test may occur. Clotted specimens are unacceptable. Extracted RNA: Only accept extracted RNA when extraction or isolation is performed in an appropriately qualified laboratory such as a CLIA-certified laboratory or a laboratory meeting equivalent requirements as determined by the CAP and/or CMS. |
| Transport container | EDTA (lavender-top) tube |
| Transport temperature | Room temperature |
| Specimen stability | Whole blood and bone marrow Room temperature: 5 days Refrigerated: 5 days Frozen: Unacceptable Extracted RNA Room temperature: Unacceptable Refrigerated: Preferred Frozen: Acceptable |
Reference Range(s)
| BCR-ABL1/ABL1 % | 0.000 |
| BCR-ABL1/ABL1 % (IS) | 0.000 |
| Interpretation | See Laboratory Report |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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