Acute Myeloid Leukemia Prognostic Panel (Normal Karyotype)

Acute Myeloid Leukemia Prognostic Panel (Normal Karyotype)

Test Code

90871
81218, 81310, 81245, 81246
CPT coding may differ dependent on payer rules which may impact prior authorization testing.
Please direct any questions regarding CPT coding to the payer being billed.
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
90871
81218, 81310, 81245, 81246
CPT coding may differ dependent on payer rules which may impact prior authorization testing.
Please direct any questions regarding CPT coding to the payer being billed.
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Acute Myeloid Leukemia Prognostic Panel (Normal Karyotype) - This testing, consisting of FLT3, NPM-1, and CEBPA, is recommended in the NCCN guidelines for determination of AML risk status in patients with cytogenetically normal AML. The presence of CEBPA gene mutations is associated with increased disease-free survival and overall survival. Mutations in NPM1 gene is a predictor of favorable prognosis and good response to induction chemotherapy. The presence of (FLT3) internal tandem duplication is associated with short disease-free survival.

Test Resources

None found for this test
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Test Details

Includes

  • CEBPA Mutation Analysis
  • NPM (Exon 12) Mutation Analysis, Cell-based
  • FLT3 Mutation Assay

Methodology

See individual tests

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

See Laboratory Report

LOINC® Codes, Performing Laboratory

Service Area must be determined
CEBPA Mutation Analysis
NPM (Exon 12) Mutation Analysis, Cell-based
FLT3 Mutation Assay

Methodology

See individual tests
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

See Laboratory Report
Service Area must be determined

Preferred Specimen(s)

5 mL whole blood collected in an EDTA (lavender-top) tube

Alternative Specimen(s)

3 mL bone marrow collected in an EDTA (lavender-top) tube • Cell pellet collected in a sterile transport tube • 5x5 mm tissue collected in a paraffin block

Minimum Volume

3 mL whole blood • 1 mL bone marrow • 2x3 mm tissue

Collection Instructions

Do not reject. Send to lab for evaluation.

Whole blood: Follow standard whole blood collection procedure. Collect 3-5 mL whole blood samples in an EDTA tube. Blood samples are shipped at room temperature or 4° C. Do not freeze whole blood. Record the draw time and date on the tube. Ship immediately to maintain sample stability.

Cell pellet: This assay is also designed for the remnant cell pellet from Cytogenetics. The cell pellet usually fixed should be transported refrigerated to the laboratory as soon as possible. Please provide a copy of the Cytogenetics report.

Transport Container

EDTA (lavender-top) tube

Transport Temperature

Room temperature

Specimen Stability

  • Whole blood and bone marrow
  • Room temperature: 7 days
  • Refrigerated: 7 days
  • Frozen: Unacceptable
  •  
  • Cell pellet
  • Room temperature: Unacceptable
  • Refrigerated: 30 days
  • Frozen: 30 days
  •  
  • Tissue
  • Room temperature: Preferred
  • Refrigerated: Acceptable
  • Frozen: Unacceptable

Setup Schedule

Service Area must be determined
5 mL whole blood collected in an EDTA (lavender-top) tube
3 mL bone marrow collected in an EDTA (lavender-top) tube • Cell pellet collected in a sterile transport tube • 5x5 mm tissue collected in a paraffin block
3 mL whole blood • 1 mL bone marrow • 2x3 mm tissue
Do not reject. Send to lab for evaluation.

Whole blood: Follow standard whole blood collection procedure. Collect 3-5 mL whole blood samples in an EDTA tube. Blood samples are shipped at room temperature or 4° C. Do not freeze whole blood. Record the draw time and date on the tube. Ship immediately to maintain sample stability.

Cell pellet: This assay is also designed for the remnant cell pellet from Cytogenetics. The cell pellet usually fixed should be transported refrigerated to the laboratory as soon as possible. Please provide a copy of the Cytogenetics report.
EDTA (lavender-top) tube
Room temperature
Whole blood and bone marrow
Room temperature: 7 days
Refrigerated: 7 days
Frozen: Unacceptable

Cell pellet
Room temperature: Unacceptable
Refrigerated: 30 days
Frozen: 30 days

Tissue
Room temperature: Preferred
Refrigerated: Acceptable
Frozen: Unacceptable
Service Area must be determined
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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