Trichomonas vaginalis RNA, Qualitative, TMA, Pap Vial
Test Code
Clinical Significance
Trichomonas vaginalis RNA, Qualitative, TMA, Pap Vial - Trichomonas vaginalis RNA, Qualitative Transcription- Mediated Amplification, Pap Vial test is a nucleic acid amplification test that uses transcription-mediated amplification for detection of T. vaginalis RNA, using a ThinPrep® or SurePath™ Pap sample as the specimen. It is used in the screening and diagnosis of trichomoniasis. When the clinical performance of this assay was evaluated, sensitivity was 100% in PreservCyt Solution liquid Pap specimens, while specificity was 98.6% [1,2].
Trichomoniasis is one of the most common sexually transmitted infections ...
Test Resources
Test Details
Methodology
Reference Range(s)
Preferred Specimen(s)
1 mL Liquid Cytology (PreservCyt®) Preservative (ThinPrep®) in Aptima® Transfer Tube or
0.5 mL SurePath™ Preservative Fluid in Aptima® Transfer Tube
Alternative Specimen(s)
20 mL PreservCyt® (ThinPrep®) • 10 mL SurePath™ Fluid
Minimum Volume
1 mL PreservCyt® (ThinPrep®) in Aptima® Transfer Tube • 0.5 mL SurePath™ Fluid in Aptima® Transfer Tube
Collection Instructions
PreservCyt® or SurePath™ material already processed for cytology or PAP are not acceptable.
Labs performing cytology: Aliquot PreservCyt® solution before performance of liquid based cytology testing.
PreservCyt®: Transfer 1 mL PreservCyt® solution into Aptima® Specimen Transfer Tube (green label).
SurePath™: SurePath™ fluid must be transferred to Aptima® tube within 4 days of collection. Transfer 0.5 mL SurePath™ solution into Aptima® Specimen Transfer Tube (green label).
Transport Container
Aptima® Vaginal Collection Kit
Transport Temperature
Room temperature
Specimen Stability
- PreservCyt® (ThinPrep®) in Aptima® Transport
- Room temperature: 14 days
- Refrigerated: 30 days
- Frozen: 12 months
- SurePath™ Fluid in Aptima® Transport
- Room temperature: 14 days
- Refrigerated: 14 days
- Frozen: Unacceptable
- PreservCyt® (ThinPrep®)
- Room temperature: 30 days
- Refrigerated: 30 days
- Frozen: 1 year
- SurePath™ Fluid
- Room temperature: 4 days
- Refrigerated: 4 days
- Frozen: Unacceptable
Reject Criteria
PreservCyt® or SurePath™ material previously processed for cytology • PreservCyt® with excess mucus
Setup Schedule
0.5 mL SurePath™ Preservative Fluid in Aptima® Transfer Tube
Labs performing cytology: Aliquot PreservCyt® solution before performance of liquid based cytology testing.
PreservCyt®: Transfer 1 mL PreservCyt® solution into Aptima® Specimen Transfer Tube (green label).
SurePath™: SurePath™ fluid must be transferred to Aptima® tube within 4 days of collection. Transfer 0.5 mL SurePath™ solution into Aptima® Specimen Transfer Tube (green label).
Room temperature: 14 days
Refrigerated: 30 days
Frozen: 12 months
SurePath™ Fluid in Aptima® Transport
Room temperature: 14 days
Refrigerated: 14 days
Frozen: Unacceptable
PreservCyt® (ThinPrep®)
Room temperature: 30 days
Refrigerated: 30 days
Frozen: 1 year
SurePath™ Fluid
Room temperature: 4 days
Refrigerated: 4 days
Frozen: Unacceptable