BRAF Mutation Analysis, Papillary Thyroid Cancer

Test Code

90477

CPT Code(s)

81210

CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

Test Code

90477

CPT Code(s)

81210

CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection is not available in your area through Quest Mobile.

Clinical Significance

BRAF Mutation Analysis, Papillary Thyroid Cancer - BRAF V600E mutations are found in 40-50% of papillary thyroid carcinoma (PTC), and much less commonly in anaplastic thyroid carcinoma or poorly differentiated carcinomas involving the thyroid. Correlation with morphologic and clinical findings is thus required.

Test Resources

None found for this test
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Test Details

Methodology

Real-Time Polymerase Chain Reaction (PCR)

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Not detected

Alternative Name(s)

B-RAF

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

Real-Time Polymerase Chain Reaction (PCR)

Assay Category

Reference Range(s)

Not detected

Alternative Name(s)

B-RAF

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

1 formalin-fixed, paraffin-embedded tissue block OR needle washing

Minimum Volume

1 tissue block or needle washing

Collection Instructions

Do not reject. Needle washings in alcohol-based fixative (e.g. Cytolyt^®) or formalin-fixed, paraffin-embedded tissue block FNA or FFPE specimens are acceptable for the assay. All specimens must be accompanied with pathology report. All FFPE specimens will be examined by a pathologist for confirmation and adequacy. Macrodissection, if warranted, is performed in the lab.

Transport Container

Paraffin-embedded tissue block

Transport Temperature

Room temperature

Specimen Stability

FNA or FFPE
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable
Needle washings
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Unacceptable

Reject Criteria

DO NOT REJECT

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

1 formalin-fixed, paraffin-embedded tissue block OR needle washing

Minimum Volume

1 tissue block or needle washing

Collection Instructions

Transport Container

Paraffin-embedded tissue block

Transport Temperature

Room temperature

Specimen Stability

FNA or FFPE
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable

Needle washings
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Unacceptable

Reject Criteria

DO NOT REJECT

Setup Schedule

Service Area must be determined

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection is not available in your area through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

The tests listed by specialty and category are a select group of tests offered. For a complete list of Quest Diagnostics tests, please adjust the filter options chosen, or refer to our Directory of Services.