Thyroid Cancer Mutation Panel
Test Code
Clinical Significance
Thyroid Cancer Mutation Panel - The thyroid mutation panel assesses for all 8 of the most common mutations or rearrangements associated with thyroid neoplasia.
The BRAF codon 600 mutation, and RET/PTC1 and RET/PTC3 rearrangements are highly associated with papillary thyroid cancer, the PAX8-PPAR{gamma} with follicular carcinomas and RAS mutations (in either HRAS, KRAS and NRAS) usually with follicular neoplasms.
See test codes 90477 (BRAF), 90473 (RET/PTC), 90474 (PPAR/PAX8), and 90479 (RAS panel) for individual components.
A thyroid cytology diagnosis code with reflex to molecular, as appropriate, is also available.
Test Resources
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Test Details
RAS Mutation Analysis, Thyroid Cancer
RET/PTC Rearrangement, Thyroid Cancer
PAX8/PPAR[gamma] Translocation, Thyroid Cancer
Methodology
Reference Range(s)
| BRAF Mutation | Not detected |
RAS Mutation Analysis, Thyroid Cancer
| HRAS Mutation | Not detected |
| KRAS Mutation | Not detected |
| NRAS Mutation | Not detected |
RET/PTC Rearrangement, Thyroid Cancer
| RETPTC1 | Not detected |
| RETPTC3 | Not detected |
PAX8/PPAR[gamma] Translocation, Thyroid Cancer
| PAX8/PPAR | Not detected |
Preferred Specimen(s)
1 formalin-fixed, paraffin-embedded tissue block or needle washing
Collection Instructions
Do not reject. Needle washings in alcohol-based fixative (e.g. Cytolyt ®) or formalin-fixed, paraffin-embedded tissue block FNA or FFPE specimens are acceptable for the assay. All specimens must be accompanied with pathology report. All FFPE specimens will be examined by a pathologist for confirmation and adequacy. Macrodissection, if warranted, is performed in the lab.
Transport Container
Fixative, slides or paraffin embedded tissue block
Transport Temperature
Room temperature
Specimen Stability
- FNA or FFPE
- Room temperature: 5 years
- Refrigerated: 5 years
- Frozen: Unacceptable
- Needle washings
- Room temperature: 30 days
- Refrigerated: 30 days
- Frozen: Unacceptable
Reject Criteria
DO NOT REJECT
Setup Schedule
1 formalin-fixed, paraffin-embedded tissue block or needle washing
Do not reject. Needle washings in alcohol-based fixative (e.g. Cytolyt ®) or formalin-fixed, paraffin-embedded tissue block FNA or FFPE specimens are acceptable for the assay. All specimens must be accompanied with pathology report. All FFPE specimens will be examined by a pathologist for confirmation and adequacy. Macrodissection, if warranted, is performed in the lab.
Room temperature: 5 years
Refrigerated: 5 years
Frozen: Unacceptable
Needle washings
Room temperature: 30 days
Refrigerated: 30 days
Frozen: Unacceptable
DO NOT REJECT