Antithrombin III Activity with Reflex to Antithrombin III Antigen

Antithrombin III Activity with Reflex to Antithrombin III Antigen

Test Code

8267
85300
8267
85300
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account or to find available tests you can order.
In-home collection is not available in your area through Quest Mobile.

Clinical Significance

Antithrombin III Activity with Reflex to Antithrombin III Antigen - Aids in characterization of Antithrombin deficiency (AT, previously referred to as Antithrombin III) which is associated with increased thrombotic risk. Type I deficiency is characterized by reduction in activity and antigen levels simultaneously. With type II deficiency, activity levels are lower in comparison to the antigen levels (dysfunctional protein). Acquired deficiency, more common than inherited defects, can occur due to: liver disease, nephrotic syndrome, heparin therapy, disseminated intra- vascular coagulation (DIC), sepsis, and L-asparaginase chemotherapy.

Anticoagulant Interference: ...

Test Resources

None found for this test
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Test Details

Includes

  • If Antithrombin III Activity is below reference interval, then Antithrombin III Antigen will be performed at an additional charge (CPT code(s): 85301).

Methodology

Chromogenic Substrate

Reference Range(s)

Antithrombin III Activity

1 day-1 month41-108 % normal
31 days-2 months50-120 % normal
3-5 months73-120 % normal
≥6 months80-135 % normal

Alternative Name(s)

AT3,Heparin Cofactor,AT III

LOINC® Codes, Performing Laboratory

Service Area must be determined
If Antithrombin III Activity is below reference interval, then Antithrombin III Antigen will be performed at an additional charge (CPT code(s): 85301).

Methodology

Chromogenic Substrate

Reference Range(s)

Antithrombin III Activity
1 day-1 month41-108 % normal
31 days-2 months50-120 % normal
3-5 months73-120 % normal
≥6 months80-135 % normal

Alternative Name(s)

AT3,Heparin Cofactor,AT III
Service Area must be determined

Preferred Specimen(s)

1 mL frozen platelet-poor plasma collected in each of two 3.2% sodium citrate (light blue-top) tubes

Minimum Volume

0.5 mL each specimen

Collection Instructions

Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collections. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice.

Transport Container

Transport tube(s)

Transport Temperature

Frozen

Specimen Stability

  • Room temperature: Unacceptable
    Refrigerated: Unacceptable
    Frozen: 30 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Grossly icteric

Setup Schedule

Service Area must be determined
1 mL frozen platelet-poor plasma collected in each of two 3.2% sodium citrate (light blue-top) tubes
0.5 mL each specimen
Please submit a separate, frozen vial for each special coagulation assay ordered. Draw blood in a light blue-top tube containing 3.2% sodium citrate, mix gently by inverting 3-4 times. Centrifuge 15 minutes at 1500 g within one hour of collections. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial(s). Freeze immediately and transport on dry ice.
Transport tube(s)
Frozen
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 30 days
Gross hemolysis • Grossly lipemic • Grossly icteric
Service Area must be determined
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account or to find available tests you can order.
In-home collection is not available in your area through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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