Protein, Total and Protein Electrophoresis

Test Code

747

CPT Code(s)

84155, 84165

Test Code

747

CPT Code(s)

84155, 84165

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Protein, Total and Protein Electrophoresis - Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).

Test Resources

None found for this test
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Test Details

Patient Preparation

Overnight fasting is preferred

Methodology

Capillary Zone Electrophoresis (CZE) • Spectrophotometry (SP)

Reference Range(s)

Protein, Total, Serum

	Male (g/dL)	Female (g/dL)
<1 Month	4.1-6.3	4.2-6.2
1-5 Months	4.7-6.7	4.4-6.6
6-11 Months	5.5-7.0	5.6-7.9
1-19 Years	6.3-8.2	6.3-8.2
≥20 Years	6.1-8.1	6.1-8.1

Protein Electrophoresis

Albumin	3.8-4.8 g/dL
Alpha-1 Globulin	0.2-0.3 g/dL
Alpha-2 Globulin	0.5-0.9 g/dL
Beta-1 Globulin	0.4-0.6 g/dL
Beta-2 Globulin	0.2-0.5 g/dL
Gamma Globulin	0.8-1.7 g/dL
Abnormal Protein Band 1	None detected
Abnormal Protein Band 2	None detected
Abnormal Protein Band 3	None detected

Alternative Name(s)

SPEP

LOINC® Codes, Performing Laboratory

Service Area must be determined

Patient Preparation

Overnight fasting is preferred

Methodology

Capillary Zone Electrophoresis (CZE) • Spectrophotometry (SP)

Reference Range(s)

Protein, Total, Serum

	Male (g/dL)	Female (g/dL)
<1 Month	4.1-6.3	4.2-6.2
1-5 Months	4.7-6.7	4.4-6.6
6-11 Months	5.5-7.0	5.6-7.9
1-19 Years	6.3-8.2	6.3-8.2
≥20 Years	6.1-8.1	6.1-8.1

Protein Electrophoresis

Albumin	3.8-4.8 g/dL
Alpha-1 Globulin	0.2-0.3 g/dL
Alpha-2 Globulin	0.5-0.9 g/dL
Beta-1 Globulin	0.4-0.6 g/dL
Beta-2 Globulin	0.2-0.5 g/dL
Gamma Globulin	0.8-1.7 g/dL
Abnormal Protein Band 1	None detected
Abnormal Protein Band 2	None detected
Abnormal Protein Band 3	None detected

Alternative Name(s)

SPEP

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

2 mL serum

Minimum Volume

1.5 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 5 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Grossly icteric • Plasma

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

2 mL serum

Minimum Volume

1.5 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 5 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Grossly icteric • Plasma

Setup Schedule

Service Area must be determined

In-home collection may be available in your area for purchase through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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