Cholesterol and HDL Cholesterol with Ratio

Cholesterol and HDL Cholesterol with Ratio

Test Code

7432
82465, 83718
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
7432
82465, 83718
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Not offered in Quest Nichols Institute - Chantilly, VA | Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano | Quest Nichols Institute -San Juan Capistrano, CA. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Cholesterol and HDL Cholesterol with Ratio -

This panel measures serum concentrations of total cholesterol and high-density lipoprotein (HDL) cholesterol and provides a calculated total cholesterol/HDL cholesterol ratio. Measurements of cholesterol in conjunction with lipoproteins are used primarily in the risk assessment of atherosclerotic cardiovascular disease (ASCVD) [1].

Elevated total cholesterol is an established risk factor for atherosclerotic coronary disease [1]. HDL cholesterol level has been inversely related to the ASCVD risk, whereas very high HDL levels may increase all-cause mortality [2]. The cholesterol/HDL cholesterol ...

Test Resources

None found for this test
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Test Details

Includes

  • Cholesterol, Total; HDL Cholesterol; Cholesterol/HDL Ratio (calculated)
  •  
  • If an HDL Cholesterol measurement is to be performed along with Triglycerides, the patient should be fasting 9-12 hours prior to collection.

Patient Preparation

If a cholesterol measurement is to be performed along with triglycerides, the patient should be fasting 9-12 hours prior to collection. The assay manufacturer Beckman Coulter advises: "N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been. . . determined to interfere with assays for. . . cholesterol, uric acid" where "NAC interference may lead to falsely low results." According to Beckman Coulter, the NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hrs and a third dose of 100 mg/kg administered over 16 hrs.

Methodology

Spectrophotometry (SP)

Reference Range(s)


Total Cholesterol 		Male
(mg/dL)		 Female
(mg/dL)
  <20 Years <170 <170
  ≥20 Years <200 <200
HDL Cholesterol    
  <20 Years >45 >45
  ≥20 Years ≥40 ≥50
Cholesterol/HDL Ratio (Calc) <5.0 <5.0

LOINC® Codes, Performing Laboratory

Service Area must be determined
Cholesterol, Total; HDL Cholesterol; Cholesterol/HDL Ratio (calculated)

If an HDL Cholesterol measurement is to be performed along with Triglycerides, the patient should be fasting 9-12 hours prior to collection.
If a cholesterol measurement is to be performed along with triglycerides, the patient should be fasting 9-12 hours prior to collection. The assay manufacturer Beckman Coulter advises: "N-Acetyl Cysteine (NAC), when administered in therapeutic concentrations (for the treatment of acetaminophen overdose), has been. . . determined to interfere with assays for. . . cholesterol, uric acid" where "NAC interference may lead to falsely low results." According to Beckman Coulter, the NAC interference should be insignificant by 12 hours after completion of the initial loading dose of an IV infusion treatment regimen consisting of an initial loading dose of 150 mg/kg administered over 1 hour, a second dose of 50 mg/kg administered over 4 hrs and a third dose of 100 mg/kg administered over 16 hrs.

Methodology

Spectrophotometry (SP)

Reference Range(s)


Total Cholesterol 		Male
(mg/dL)		 Female
(mg/dL)
  <20 Years <170 <170
  ≥20 Years <200 <200
HDL Cholesterol    
  <20 Years >45 >45
  ≥20 Years ≥40 ≥50
Cholesterol/HDL Ratio (Calc) <5.0 <5.0
Service Area must be determined

Preferred Specimen(s)

1 mL serum

Alternative Specimen(s)

Plasma collected in: Sodium heparin (green-top) tube or lithium heparin (green-top) tube

Minimum Volume

0.5 mL

Collection Instructions

Centrifuge within 30-60 minutes following collection

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Serum
  • Room temperature: 48 hours
  • Refrigerated: 7 days
  • Frozen: 28 days
  •  
  • Plasma
  • Room temperature: 48 hours
  • Refrigerated: 48 hours
  • Frozen: 28 days

Reject Criteria

Gross hemolysis • Moderate to gross icterus • Anticoagulants other than heparin

Setup Schedule

Service Area must be determined
1 mL serum
Plasma collected in: Sodium heparin (green-top) tube or lithium heparin (green-top) tube
0.5 mL
Centrifuge within 30-60 minutes following collection
Transport tube
Room temperature
Serum
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days

Plasma
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: 28 days
Gross hemolysis • Moderate to gross icterus • Anticoagulants other than heparin
Service Area must be determined
Not offered in Quest Nichols Institute - Chantilly, VA | Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano | Quest Nichols Institute -San Juan Capistrano, CA. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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