Immunoassay (IA)
Specimen requirements | |
|---|---|
| Patient preparation | Overnight fasting is required. High carbohydrate diet for 3 days before test. Patient NPO after midnight; glucose solution 1.75 g/kg body weight (maximum dose 75 g) orally administered after collection of fasting specimen. |
| Preferred specimen(s) | 1 mL serum per specimen |
| Minimum volume | 0.5 mL per specimen |
| Collection instructions | Draw fasting specimen. Collect 1 mL serum for each timed specimen post glucose dose. All tubes must be clearly marked with time drawn. Submit all tubes with one test requisition. Reference ranges available: Fasting and 30, 60, 90, 120, 150, 180, 240 and 300 minutes post glucose. Unable to add INSULIN testing unless sample was submitted frozen. |
| Transport container | Transport tube(s) |
| Transport temperature | Refrigerated (cold packs) |
| Specimen stability | Room temperature: Unacceptable Refrigerated: 7 days Frozen: 28 days |
| Reject criteria | Hemolysis • Plasma • Grossly icteric |
| Fasting | 2.0-18.4 uIU/mL |
| 30 Minutes Post Glucose | 6.0-86.0 uIU/mL |
| 60 Minutes Post Glucose | 8.0-112.0 uIU/mL |
| 90 Minutes Post Glucose | 5.0-68.0 uIU/mL |
| 120 Minutes Post Glucose | 5.0-55.0 uIU/mL |
| 150 Minutes Post Glucose | 3.0-46.0 uIU/mL |
| 180 Minutes Post Glucose | 3.0-20.0 uIU/mL |
| 240 Minutes Post Glucose | <15.0 uIU/mL |
| 300 Minutes Post Glucose | <8.0 uIU/mL |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
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