Methotrexate
Test code
CPT code(s)*
Test resources
Details
Methodology
Immunoassay (IA)
Specimen requirements | |
|---|---|
| Patient preparation | Collect 24, 48, or 72 hours after dose |
| Preferred specimen(s) | 1 mL frozen serum collected in a red-top tube (no gel) |
| Alternative specimen(s) | Plasma collected in: EDTA (lavender-top) tube, fluoride oxalate (gray-top) tube, sodium or lithium heparin (green-top) tube |
| Minimum volume | 0.2 mL |
| Collection instructions | Protect from light. Record hours from last dose on specimen container and in the remark column on the test requisition. |
| Transport container | Transport tube |
| Transport temperature | Frozen |
| Specimen stability | Room temperature: 24 hours Refrigerated: Unacceptable Frozen: 5 days |
| Reject criteria | Serum separator tube (SST) or any other tube with additives • Received thawed |
Reference Range(s)
| <5.00 umol/L at 24 hours |
| <0.50 umol/L at 48 hours |
| <0.20 umol/L at 72 hours |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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