Culture, Anaerobic Bacteria with Gram Stain

Culture, Anaerobic Bacteria with Gram Stain

Test Code

4469
87075, 87205
4469
87075, 87205
Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account.
In-home collection is not available in your area through Quest Mobile.

Clinical Significance

Culture, Anaerobic Bacteria with Gram Stain - Anaerobic bacteria cause a variety of human infections. The results of anaerobic cultures assist the clinician with diagnosis and treatment of patients with anaerobic infections.

Test Resources

None found for this test
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Test Details

Includes

  • If culture is positive, identification will be performed at an additional charge (CPT code(s): 87076 or 87143 or 87140 or 87149). Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87185 or 87186).

Methodology

Direct Microscopy • Bacterial Culture • Anaerobic Isolation and Identification Procedures • Phenotypic • Matrix Assisted Laser Desorption Ionization-Time of Flight • Sequencing

Reference Range(s)

No growth

Alternative Name(s)

Deep Wound,Body Fluid Culture,Abscess Culture

LOINC® Codes, Performing Laboratory

Service Area must be determined
If culture is positive, identification will be performed at an additional charge (CPT code(s): 87076 or 87143 or 87140 or 87149). Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87185 or 87186).

Methodology

Direct Microscopy • Bacterial Culture • Anaerobic Isolation and Identification Procedures • Phenotypic • Matrix Assisted Laser Desorption Ionization-Time of Flight • Sequencing

Reference Range(s)

No growth

Alternative Name(s)

Deep Wound,Body Fluid Culture,Abscess Culture
Service Area must be determined

Preferred Specimen(s)

3 mL or 3 grams abscess or deep wound, tissue, normally sterile body fluids other than blood, bronchoscopic specimens obtained by the Double Lumen Brush technique, suprapubic urine aspirates obtained from bladder, Nephrostomy tube or suprapubic catheterization, aspirated pus from deep wound/abscess/tympanocentesis fluid, genital specimens from these sites only: -cul-de-sac aspiration, -culdocentesis -percutaneous transfundal aspiration, fallopian tube, surgical specimens, exudates collected in an anaerobic transport gel vial for fluid and tissue specimens or an anaerobic transport swab with Amies gel (blue-cap) or ESwab (white-cap)

Alternative Specimen(s)

⁠⁠⁠⁠⁠⁠⁠(2 swabs) abscess or deep wound • Normally sterile body fluids other than blood • Bronchoscopic specimens obtained by the Double Lumen Brush technique • Suprapubic urine aspirates obtained from bladder • Nephrostomy tube or suprapubic catheterization • Aspirated pus from deep wound/abscess/tympanocentesis fluid • Genital specimens from these sites only: -cul-de-sac aspiration, -culdocentesis -percutaneous transfundal aspiration • Fallopian tube • Surgical specimens • Exudates collected in Amies gel (blue-cap)/ESwab (white-cap)

Minimum Volume

1 mL or 1 gram

Collection Instructions

⁠⁠⁠⁠⁠⁠⁠Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for gram stain OR submit one anaerobic transport swab with one air dried smear or one ESwab (white-cap) for both culture and gram stain.
Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.

Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe. Purulent sterile body fluids not submitted in an anaerobic transport tube may be accepted for culture if received within 8 hours of collection.

Transport Container

Anaerobic transport gel vial for fluid and tissue specimens • Anaerobic transport swab with Aimes gel (blue-cap) or ESwab (white-cap)

Transport Temperature

Room temperature

Specimen Stability

  • Anaerobic transport media
  • Room temperature: 72 hours
  • Refrigerated: 72 hours
  • Frozen: Unacceptable
  •  
  • ESwab or Amies gel swab
  • Room temperature: 48 hours
  • Refrigerated: 48 hours
  • Frozen: Unacceptable
  •  

Reject Criteria

Specimens not in an anaerobic transport medium • Expired transport media • Throat, nasopharyngeal, sputum or bronchoscopic specimens • Specimens not collected by Double Lumen Brush technique • Stool and rectal swabs • Urine-voided or catheter • Vaginal and cervical • Specimens from sites contaminated with intestinal contents • Superficial or fresh wounds • External ear • External eye • Intrauterine device • Tracheal aspirates

Setup Schedule

Service Area must be determined
3 mL or 3 grams abscess or deep wound, tissue, normally sterile body fluids other than blood, bronchoscopic specimens obtained by the Double Lumen Brush technique, suprapubic urine aspirates obtained from bladder, Nephrostomy tube or suprapubic catheterization, aspirated pus from deep wound/abscess/tympanocentesis fluid, genital specimens from these sites only: -cul-de-sac aspiration, -culdocentesis -percutaneous transfundal aspiration, fallopian tube, surgical specimens, exudates collected in an anaerobic transport gel vial for fluid and tissue specimens or an anaerobic transport swab with Amies gel (blue-cap) or ESwab (white-cap)
⁠⁠⁠⁠⁠⁠⁠(2 swabs) abscess or deep wound • Normally sterile body fluids other than blood • Bronchoscopic specimens obtained by the Double Lumen Brush technique • Suprapubic urine aspirates obtained from bladder • Nephrostomy tube or suprapubic catheterization • Aspirated pus from deep wound/abscess/tympanocentesis fluid • Genital specimens from these sites only: -cul-de-sac aspiration, -culdocentesis -percutaneous transfundal aspiration • Fallopian tube • Surgical specimens • Exudates collected in Amies gel (blue-cap)/ESwab (white-cap)

1 mL or 1 gram

⁠⁠⁠⁠⁠⁠⁠Tissues and fluids are superior to a swab specimen. If swabs must be used, collect 2 anaerobic transport swabs, one for culture and one for gram stain OR submit one anaerobic transport swab with one air dried smear or one ESwab (white-cap) for both culture and gram stain.
Specimens not listed as acceptable may be processed for anaerobic culture only after consultation with a Quest Microbiology Scientific Director.

Do not submit syringe with needle attached. If submitting syringe, remove needle, expel air and cap syringe. Purulent sterile body fluids not submitted in an anaerobic transport tube may be accepted for culture if received within 8 hours of collection.
Anaerobic transport gel vial for fluid and tissue specimens • Anaerobic transport swab with Aimes gel (blue-cap) or ESwab (white-cap)
Room temperature
Anaerobic transport media
Room temperature: 72 hours
Refrigerated: 72 hours
Frozen: Unacceptable

ESwab or Amies gel swab
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Specimens not in an anaerobic transport medium • Expired transport media • Throat, nasopharyngeal, sputum or bronchoscopic specimens • Specimens not collected by Double Lumen Brush technique • Stool and rectal swabs • Urine-voided or catheter • Vaginal and cervical • Specimens from sites contaminated with intestinal contents • Superficial or fresh wounds • External ear • External eye • Intrauterine device • Tracheal aspirates
Service Area must be determined
Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account.
In-home collection is not available in your area through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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