Borrelia burgdorferi DNA, Qualitative Real-Time PCR, Miscellaneous
Test Code
Clinical Significance
Borrelia burgdorferi DNA, Qualitative Real-Time PCR, Miscellaneous - The diagnosis of Lyme disease is most often made by history and clinical examination combined with exposure in endemic areas. Amplification of Borrelia genomic DNA from blood, fluids or tissues can support the diagnosis. A negative result does not rule out Borrelia infection and appropriate serology testing may be clinically warranted.
Test Resources
Test FAQ
Lyme Disease TestingClinical Focus
Lyme Disease: Laboratory Support of Diagnosis and ManagementTest Details
Methodology
Reference Range(s)
Alternative Name(s)
Preferred Specimen(s)
3 mL whole blood collected in an EDTA (lavender-top) tube or
3 mL CSF or synovial fluid collected in a sterile plastic leak-proof container
Alternative Specimen(s)
Whole blood collected in: ACD (yellow-top) tube
Minimum Volume
0.5 mL
Transport Container
Whole blood: EDTA (lavender-top) tube
CSF or synovial fluid: Sterile leak-proof container
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 daysCSF or synovial fluid
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria
Lithium heparin (green-top) tube • Sodium heparin (green-top) tube
Setup Schedule
3 mL CSF or synovial fluid collected in a sterile plastic leak-proof container
CSF or synovial fluid: Sterile leak-proof container
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
CSF or synovial fluid
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days