Culture, Blood
Test Code
Clinical Significance
Culture, Blood - Bacterial sepsis constitutes one of the most serious infectious diseases. The detection of microorganisms in a patient's blood has importance in the diagnosis and prognosis of endocarditis, septicemia, or chronic bacteremia.
Test Resources
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Test Details
If culture is positive, identification will be performed at an additional charge (CPT code(s): 87076 or 87106 or 87077 or 87140 or 87143 or 87147 or 87149).
Antibiotic susceptibilities are only performed when appropriate (CPT code(s): 87181 or 87184 or 87185 or 87186).
Methodology
Alternative Name(s)
Preferred Specimen(s)
Adults: 8-10 mL of blood collected in one of each BacT/ALERT FA PLUS (Pale Green) and BacT/ALERT FN PLUS (Orange) bottles
Neonates and children 1 to 6 years: 1-3 mL blood collected in a BacT/ALERT PF PLUS (Yellow) bottle
Children weighing 30-80 lbs: 3 mL blood collected in a BacT/ALERT PF PLUS (Yellow) bottle or
8-10 mL blood collected in one of each BacT/ALERT FA PLUS (Pale Green) and BacT/ALERT FN PLUS (Orange) bottles
Alternative Specimen(s)
Bone marrow cultures: For adults, bone marrow collected in one of each BacT/ALERT FA PLUS (Pale Green) and BacT/ALERT FN PLUS (Orange) bottles • Blood culture bottles compatible with local automated instrumentation
Minimum Volume
3 mL BacT/ALERT FA PLUS (Pale Green) and BacT/ALERT FN PLUS (Orange) bottles • 0.5 mL BacT/ALERT PF PLUS (Yellow) bottle
Collection Instructions
Do not pre-incubate bottles prior to shipment
Transport Container
BacT/ALERT FA PLUS (Pale green) and BacT/ALERT FN PLUS (Orange) bottles or
BacT/ALERT PF PLUS (Yellow) bottle
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 48 hours
- Refrigerated: Unacceptable
- Frozen: Unacceptable
Reject Criteria
Blood specimens collected in EDTA, sodium citrate or anticoagulants such as SPS or heparin
Setup Schedule
Neonates and children 1 to 6 years: 1-3 mL blood collected in a BacT/ALERT PF PLUS (Yellow) bottle
Children weighing 30-80 lbs: 3 mL blood collected in a BacT/ALERT PF PLUS (Yellow) bottle or
8-10 mL blood collected in one of each BacT/ALERT FA PLUS (Pale Green) and BacT/ALERT FN PLUS (Orange) bottles
BacT/ALERT PF PLUS (Yellow) bottle
Refrigerated: Unacceptable
Frozen: Unacceptable