Epidermal Antibodies with Reflex to Titer

Test Code

37097

CPT Code(s)

86255 (x2)

Test Code

37097

CPT Code(s)

86255 (x2)

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Epidermal Antibodies with Reflex to Titer - The detection of anti-skin antibodies aids in the diagnosis of chronic vesicular-bullous diseases including pemphigus, pemphigoid, cicatricial pemphigoid, and epidermolysis bullosa acquisita (EBA).
Epithelial intercellular antibodies are diagnostic for pemphigus and occur in over 90% of sera from all active forms. Antibodies to basement membrane antigens of stratified squamous epithelium occur in about 70% of active bullous pemphigoid, 50% of vesicular pemphigoid and EBA and 10% of cicatricial pemphigoid patients.

Test Resources

None found for this test
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Test Details

Includes

Intercellular and Basement Antibody Screens with Reflex to Titers
If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).

Methodology

Immunofluorescence Assay (IFA)

Reference Range(s)

Intercellular Substance Ab	Negative
Titer	<1:10
Basement Membrane Zone Ab	Negative
Titer	<1:10

LOINC® Codes, Performing Laboratory

Service Area must be determined

Includes

Intercellular and Basement Antibody Screens with Reflex to Titers

If Intercellular Substance Antibody Screen is positive, then Intercellular Substance Antibody Titer will be performed at an additional charge (CPT code(s): 86256).
If Basement Membrane Zone Antibody Screen is positive, then Basement Membrane Zone Titer will be performed at an additional charge (CPT code(s): 86256).

Methodology

Immunofluorescence Assay (IFA)

Reference Range(s)

Intercellular Substance Ab	Negative
Titer	<1:10
Basement Membrane Zone Ab	Negative
Titer	<1:10

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

0.5 mL serum

Minimum Volume

0.1 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen -20° C: 30 days
Frozen -70° C: Indefinite

Reject Criteria

Gross hemolysis • Grossly lipemic • Gross icterus

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

0.5 mL serum

Minimum Volume

0.1 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen -20° C: 30 days
Frozen -70° C: Indefinite

Reject Criteria

Gross hemolysis • Grossly lipemic • Gross icterus

Setup Schedule

Service Area must be determined

In-home collection may be available in your area for purchase through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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