ANAlyzeR™ ANA, IFA with Reflex Titer/Pattern, Systemic Autoimmune Panel 1

Test code

36378

CPT code(s)*

86038, 86255, 86235 (x9), 86160 (x2), 86147 (x3), 86146 (x3), 83520 (x4), 86200, 86376

In-home collection is not available in your area through Quest Mobile.

Clinical significance

ANAlyzeR™ ANA, IFA with Reflex Titer/Pattern, Systemic Autoimmune Panel 1 - This panel can be helpful in the diagnosis of systemic rheumatic disorders and other autoimmune diseases. Although the test for antinuclear antibodies (ANA) is strongly associated with systemic lupus erythematosus (SLE), the laboratory evaluation for individuals with clinical suspicion of a variety of autoimmune diseases often begins with an ANA screen. This panel simultaneously tests for ANA (using immunofluorescence) as well as the important specific autoantibodies associated with a positive ANA. It also includes testing for complement proteins, rheumatoid arthritis, Sjogren's…

Test resources

Details

Test details

The information below details the components of this test. For overall setup schedule, see Performing lab & setup schedule.

Included components

Test code	Test name	Orderable separately	Setup schedule
249	ANA Screen,IFA, with Reflex to Titer and Pattern	Yes	Set up: Mon-Fri (Day shift and Night shift); Report available: 4 days
36552	B2 GLYCOPROTEIN I (IGA)AB	Yes	Not provided
36554	B2 GLYCOPROTEIN I (IGG)AB	Yes	Not provided
36553	B2 GLYCOPROTEIN I (IGM)AB	Yes	Not provided
4661	Cardiolipin Antibody (IgA)	Yes	Set up: Mon-Fri (All shifts); Report available: 2 days
4662	Cardiolipin Antibody (IgG)	Yes	Set up: Mon-Fri (All shifts); Report available: 2 days
4663	Cardiolipin Antibody (IgM)	Yes	Set up: Mon-Fri (All shifts); Report available: 2 days
16088	Centromere B Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
34088	Chromatin (Nucleosomal) Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
351	Complement Component C3c	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
353	Complement Component C4c	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
11173	Cyclic Citrullinated Peptide (CCP) Antibody (IgG)	Yes	Set up: Mon-Fri (All shifts); Report available: 3 days
37092	DNA (DS) ANTIBODY,CRITHIDIA, IFA W/REFL	Yes	Not provided
5810	Jo-1 Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 3 days
13238	Mutated Citrullinated Vimentin (MCV) Antibody	Yes	Set up: Mon-Sat; Report available: 3 days
15682	Rheumatoid Factor (IgA)	Yes	Set up: Mon-Sat; Report available: 3 days
15683	Rheumatoid Factor (IgG)	Yes	Set up: Mon-Sat; Report available: 3 days
17743	Rheumatoid Factor (IgM)	Yes	Set up: Mon-Sat; Report available: 3 days
19887	RNP Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
4942	Scleroderma Antibody (Scl-70)	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
38568	Sjogren's Antibody (SS-A)	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
38569	Sjogren's Antibody (SS-B)	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
37923	Sm Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
38567	Sm/RNP Antibody	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day
5081	Thyroid Peroxidase Antibodies (TPO)	Yes	Set up: Mon-Sat (All shifts); Report available: 1 day

Includes

ANA Screen,IFA, with Reflex to Titer and Pattern
DNA (ds) Antibody, Crithidia IFA with Reflex to Titer
Chromatin (Nucleosomal) Antibody
Sm Antibody
Sm/RNP Antibody
RNP Antibody
Sjogren's Antibodies (SS-A, SS-B)
Scleroderma Antibody (Scl-70)
Jo-1 Antibody
Centromere B Antibody
Complement Component C3c and C4c
Cardiolipin Antibodies (IgA, IgG, IgM)
Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Rheumatoid Factor (IgA, IgG, IgM)
Cyclic Citrullinated Peptide (CCP) Antibody (IgG)
Mutated Citrullinated Vimentin (MCV) Antibody
Thyroid Peroxidase Antibodies (TPO)

If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

If the DNA (ds) Antibody Screen is positive, then DNA (ds) Antibody Titer will be performed at an additional charge (CPT code(s): 86256).

Methodology

See individual tests

Alternative name(s)

Expanded ANA Antibodies,systemic lupus erythematosus,ANA and Systemic Autoimmunity,Lupus,ANA and Expanded AI Testing,Comprehensive AI Testing,Autoimmune Disorders,Systemic Autoimmune Disorder,Early Systemic Autoimmune Disease

Specimen requirements
Patient preparation	Overnight fasting is preferred
Preferred specimen(s)	9 mL serum
Minimum volume	6 mL
Collection instructions	Draw three, full, 8.5 mL serum separator tubes. Allow the blood to clot for at least 30 minutes, but not longer than 1 hour before centrifugation. Centrifuge each tube for 15 minutes at 1250 to 1600 RCF. If drawn in red-top (no gel) tubes, transfer serum to transport tube(s). Place sample(s) on cold packs or in refrigerator. Sample is stable 4 days at 2-8° C (refrigerated).
Transport container	Transport tube
Transport temperature	Refrigerated (cold packs)
Specimen stability	Room temperature: Unacceptable Refrigerated: 7 days Frozen: 8 days
Reject criteria	See individual tests

Reflex tests

Test code	Test name	Orderable separately	Reflex criteria
%36209	ANTINUCLEAR ANTIBODIES TITER AND PATTERN	No	If ANA Screen, IFA is Positive, then ANA Titer and Pattern will be performed.
%36731	DNA AB (DS) CRITHIDIA TITER	No	If DNA (ds) Antibody Screen is Positive, then DNA (ds) Antibody Titer will be performed.

Reference range(s)

See Laboratory Report

LOINC® codes

The Result and LOINC® information listed below should not be used for electronic interface maintenance with Quest Diagnostics. Please contact the Quest Diagnostics Connectivity Help Desk for more information at 800-697-9302.

NOTE: The codes listed in the table are not orderable Test Codes

Result code	Result name	LOINC code	Component name
45000400	CARDIOLIPIN AB (IGG)	3181-5	Cardiolipin Ab.IgG
45000500	CARDIOLIPIN AB (IGM)	3182-3	Cardiolipin Ab.IgM
45000600	CARDIOLIPIN AB (IGA)	5076-5	Cardiolipin Ab.IgA
45004300	CYCLIC CITRULLINATED PEPTIDE (CCP) AB (IGG)	33935-8	Cyclic citrullinated peptide Ab.IgG
45009300	COMPLEMENT COMPONENT C3C	4485-9	Complement C3
45009600	COMPLEMENT COMPONENT C4C	4498-2	Complement C4
45029100	THYROID PEROXIDASE ANTIBODIES	8099-4	Thyroperoxidase Ab
45029400	DNA AB (DS) CRITHIDIA,IFA	6457-6	DNA double strand Ab
45030401	B2 GLYCOPROTEIN I (IGG)AB	16135-6	Beta 2 glycoprotein 1 Ab.IgG
45030402	B2 GLYCOPROTEIN I (IGM)AB	16136-4	Beta 2 glycoprotein 1 Ab.IgM
45030403	B2 GLYCOPROTEIN I (IGA)AB	21108-6	Beta 2 glycoprotein 1 Ab.IgA
45060420	ANA SCREEN, IFA	42254-3	Nuclear Ab
45061015	SJOGREN'S ANTIBODY (SS-A)	33569-5	Sjogrens syndrome-A extractable nuclear Ab
45061020	SJOGREN'S ANTIBODY (SS-B)	45142-7	Sjogrens syndrome-B extractable nuclear Ab
45061025	SM ANTIBODY	43182-5	Smith extractable nuclear Ab
45061030	SM/RNP ANTIBODY	14030-1	Smith extractable nuclear Ab+Ribonucleoprotein extractable nuclear Ab
45061035	RNP ANTIBODY	29374-6	Ribonucleoprotein extractable nuclear Ab
45061040	SCL-70 ANTIBODY	26975-3	SCL-70 extractable nuclear Ab
45061045	JO-1 ANTIBODY	35333-4	Jo-1 extractable nuclear Ab
45061050	CENTROMERE B ANTIBODY	53982-5	Centromere protein B Ab
45061055	CHROMATIN (NUCLEOSOMAL) ANTIBODY	34416-8	Nucleosome Ab
85999717	RHEUMATOID FACTOR (IGA)	33313-8	Rheumatoid factor.IgA
85999718	RHEUMATOID FACTOR (IGG)	33314-6	Rheumatoid factor.IgG
86004808	RHEUMATOID FACTOR (IGM)	11573-3	Rheumatoid factor.IgM
86038212	MUTATED CITRULLINATED VIMENTIN (MCV) AB	54022-9	Mutated citrullinated vimentin Ab

ANTINUCLEAR ANTIBODIES TITER AND PATTERN

Result code	Result name	LOINC code	Component name
45060150	ANA PATTERN	13068-2	Nuclear Ab pattern
45060160	ANA TITER	5048-4	Nuclear Ab
86025609	ANA TITER	5048-4	Nuclear Ab
86025610	ANA PATTERN	13068-2	Nuclear Ab pattern
86025611	ANA TITER	5048-4	Nuclear Ab
86025612	ANA PATTERN	13068-2	Nuclear Ab pattern

DNA AB (DS) CRITHIDIA TITER

Result code	Result name	LOINC code	Component name
45029500	DNA AB (DS) CRITHIDIA TITER	34187-5	DNA double strand Ab

Performing lab & setup schedule

Performing laboratory

Quest Diagnostics - East Region

Setup schedule

Information not provided in East (Eastern PA, MD, DE, VA, NY, NJ, Southern CT) – Contact lab or Customer service to determine turnaround time

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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