Leukemia/Lymphoma Evaluation
Test Code
Clinical Significance
Leukemia/Lymphoma Evaluation - This panel may be used in the initial evaluation of patients with clinical suspicion of hematologic neoplasms. Immunophenotyping by flow cytometry can aid in the diagnosis and classification of leukemia and lymphoma [1]. Subsequent testing of additional cell surface antigens may be needed to help establish a diagnosis.
Leukemias and lymphomas are heterogenous morphologically and clinically. Identification of cell surface markers (T cell, B cell, progenitor, and myeloid markers) by flow cytometry can help assign a cell lineage to neoplastic cells. Accurate immunotyping aids in the diagnosis, ...
Test Resources
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Test Details
Additional markers may be performed based on the pathologist review. These markers will be performed at an additional charge (CPT code(s): 88185 for each additional marker).
Methodology
This test code is for non-New York patient testing. For New York patient testing, use test code 37340.
Reference Range(s)
Alternative Name(s)
Preferred Specimen(s)
Peripheral blood: 4 mL collected in a sodium heparin (green-top) tube, ACD-A (yellow-top) tube, or EDTA (lavender-top) tube or
Bone marrow: 3 mL collected in a sodium heparin (green-top) tube, ACD-A (yellow-top) tube,or EDTA (lavender-top) tube or
Tissue or body fluids: Any type is acceptable
Alternative Specimen(s)
Bone marrow: Pediatric heparin tube
Minimum Volume
3 mL peripheral blood • 1 mL bone marrow
Collection Instructions
Peripheral blood and bone marrow: The tube must be kept at room temperature and shipped to the lab immediately.
Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) Ship tissue in a sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.
Body fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. (A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately.
A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.
Transport Container
See Collection Instructions
Transport Temperature
Peripheral blood and bone marrow: Room temperature
Tissue and body fluids: Refrigerate (cold packs)
Specimen Stability
- Room temperature: 72 hours (may extend to 5 days depending on cell viability)
- Refrigerated: Not established
- Frozen: Not established
Reject Criteria
See Collection Instructions
Setup Schedule
Bone marrow: 3 mL collected in a sodium heparin (green-top) tube, ACD-A (yellow-top) tube,or EDTA (lavender-top) tube or
Tissue or body fluids: Any type is acceptable
Tissue: Any tissue type is acceptable. Tissue size is dependent upon leukocyte cellularity. (The tissue is disaggregated into single cells so that a minimum of 50,000 cells of interest are harvested.) Ship tissue in a sterile plastic container with RPMI 1640 enriched with FBS (10% FBS RPMI). Absolutely no fixative should be added. Refrigerate and ship immediately.
Body fluids: Any body fluid is acceptable. Sample size is dependent upon cellularity of the sample. (A minimum of 50,000 cells of interest in total volume of fluid). Place fluid in sterile plastic container. Absolutely no fixative should be added. Refrigerate and ship immediately.
A clinical indication and specimen source are required with each specimen. If possible, submit CBC results with differential or an EDTA tube of peripheral blood.
Do not freeze and do not place in fixative.
Because of the critical nature of these specimens, the laboratory will attempt to process all specimens received, regardless of age of specimen.
Tissue and body fluids: Refrigerate (cold packs)
Refrigerated: Not established
Frozen: Not established