Kleihauer-Betke Stain
Test Code
Clinical Significance
Kleihauer-Betke Stain - This test quantifies the volume of fetal-maternal hemorrhage (FMH). In Rh-positive mothers it is used solely to establish the volume of fetal hemorrhage into the maternal circulation. In Rh negative mothers it is also used to determine if an increased dose of Rh immune globulin (RhlG, anti-D antibody) is necessary. Based on an assumed total maternal circulatory volume of 5,000 mL, the FMH is calculated by multiplying the % Fetal Cells by 50. Please refer to the most current AABB Technical manual for RhlG dosage information.
Note: This test is not be ordered for Hemoglobin F Differential Staining.
Test Resources
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Test Details
Methodology
Reference Range(s)
Preferred Specimen(s)
3 mL whole blood collected in an EDTA (lavender-top) tube
Minimum Volume
1 mL
Transport Container
EDTA (lavender-top) tube
Transport Temperature
Refrigerated (cold packs)
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 14 days
- Frozen: Unacceptable
Reject Criteria
Hemolysis • Clotted
Setup Schedule
Refrigerated: 14 days
Frozen: Unacceptable