HIV-1 RNA, Quantitative, PCR with Reflex to Genotype

Test Code

34471

CPT Code(s)

87536

CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

Test Code

34471

CPT Code(s)

87536

CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

In-home collection is not available in your area through Quest Mobile.

Clinical Significance

HIV-1 RNA, Quantitative, PCR with Reflex to Genotype - Measurement of HIV-1 plasma levels (viral load) provides a direct assessment of viremia and should be used in conjunction with CD4+ T-cell counts. HIV-1 RNA, quantitation is useful in patients to assess prognosis, monitor progression of HIV-1 infection, determine when to initiate therapy, and monitor the effectiveness of antiretroviral therapy.

Test Resources

Test FAQ

HIV-1 Genotype

Test Guide

HIV Infection: Laboratory Testing for Diagnosis and Management

Test Details

Includes

If HIV-1 RNA, Quantitative, Real-Time PCR viral load is ≥400 copies/mL, then HIV-1 Genotype will be performed at an additional charge (CPT code(s): 87900, 87901).

Methodology

Real-Time Polymerase Chain Reaction

Reference Range(s)

HIV-1 RNA, QN, PCR	Not detected	copies/mL
HIV-1 RNA, QN, PCR	Not detected	Log copies/mL
HIV-1 Genotype	See Laboratory Report

Alternative Name(s)

HIV-1 RNA, HIV Quant, HIV Genotyping

LOINC® Codes, Performing Laboratory

Service Area must be determined

Includes

If HIV-1 RNA, Quantitative, Real-Time PCR viral load is ≥400 copies/mL, then HIV-1 Genotype will be performed at an additional charge (CPT code(s): 87900, 87901).

Methodology

Real-Time Polymerase Chain Reaction

Reference Range(s)

HIV-1 RNA, QN, PCR	Not detected	copies/mL
HIV-1 RNA, QN, PCR	Not detected	Log copies/mL
HIV-1 Genotype	See Laboratory Report

Alternative Name(s)

HIV-1 RNA, HIV Quant, HIV Genotyping

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

2 mL plasma collected in each of two EDTA (lavender-top) tubes

Alternative Specimen(s)

Plasma collected in each of two PPT (white-top) tubes

Minimum Volume

1 mL (x2)

Collection Instructions

Samples submitted in primary tube will be rejected. Freshly drawn specimens (whole blood) may be stored at 2-25° C for up to 24 hours prior to centrifugation. Separate plasma from cells within 24 hours of collection by centrifugation and immediately transfer to a transport tube. Follow manufacturer's instructions for collection tube handling.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 42 days

Reject Criteria

Gross hemolysis • Lipemia • Samples received in primary collection tube

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

2 mL plasma collected in each of two EDTA (lavender-top) tubes

Alternative Specimen(s)

Plasma collected in each of two PPT (white-top) tubes

Minimum Volume

1 mL (x2)

Collection Instructions

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

Room temperature: Unacceptable
Refrigerated: 6 days
Frozen: 42 days

Reject Criteria

Gross hemolysis • Lipemia • Samples received in primary collection tube

Setup Schedule

Service Area must be determined

In-home collection is not available in your area through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

The tests listed by specialty and category are a select group of tests offered. For a complete list of Quest Diagnostics tests, please adjust the filter options chosen, or refer to our Directory of Services.