Toxoplasma gondii DNA, Qualitative Real-Time PCR

Toxoplasma gondii DNA, Qualitative Real-Time PCR

Test Code

34451
87798
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
34451
87798
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.
Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account.
In-home collection is not available in your area through Quest Mobile.

Clinical Significance

Toxoplasma gondii DNA, Qualitative Real-Time PCR - Toxoplasma gondii, an obligate intracellular parasite, is an important opportunistic pathogen of immunosuppressed patients. In AIDS patients and transplant patients, this infection may result in a life-threatening encephalitis. T. gondii can also cause a fatal infection of the fetus if an infection is acquired during pregnancy. Fetal death or major abnormalities such as blindness and mental retardation may occur when infection is acquired during the first trimester. PCR methods may be useful in identifying T. gondii in CSF of immunosuppressed patients or in the amniotic fluid of mothers thought to be recently infected.

Test Resources

None found for this test
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Test Details

Methodology

Real-Time Polymerase Chain Reaction

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Not Detected

Alternative Name(s)

Toxoplasmosis, T gondii DNA

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

Real-Time Polymerase Chain Reaction
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Not Detected

Alternative Name(s)

Toxoplasmosis, T gondii DNA
Service Area must be determined

Preferred Specimen(s)

1 mL amniotic fluid or CSF collected in a sterile leak-proof container or
3 cubic mm tissue collected in a sterile leak-proof container

Alternative Specimen(s)

Serum • Whole blood or plasma collected in: EDTA (lavender-top) or ACD (yellow-top) tube • Amniotic fluid supernatant collected in: Sterile leak-proof container • 0.2 mL vitreous fluid collected in a sterile leak-proof container

Minimum Volume

3 cubic mm tissue • 0.2 mL vitreous fluid • 0.3 mL all other sample types

Collection Instructions

Raw vitreous fluid specimens: Do not dilute collected specimen with additional saline

Transport Container

Transport tube

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

  • Whole blood
  • Room temperature: 48 hours
  • Refrigerated: 7 days
  • Frozen: Unacceptable
  •  
  • All other samples
  • Room temperature: 48 hours
  • Refrigerated: 7 days
  • Frozen: 30 days

Reject Criteria

Hemolyzed whole blood

Setup Schedule

Service Area must be determined
1 mL amniotic fluid or CSF collected in a sterile leak-proof container or
3 cubic mm tissue collected in a sterile leak-proof container
Serum • Whole blood or plasma collected in: EDTA (lavender-top) or ACD (yellow-top) tube • Amniotic fluid supernatant collected in: Sterile leak-proof container • 0.2 mL vitreous fluid collected in a sterile leak-proof container
3 cubic mm tissue • 0.2 mL vitreous fluid • 0.3 mL all other sample types
Raw vitreous fluid specimens: Do not dilute collected specimen with additional saline
Transport tube
Refrigerated (cold packs)
Whole blood
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: Unacceptable

All other samples
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days
Hemolyzed whole blood
Service Area must be determined
Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.
Please provide SERVICE AREA INFORMATION to confirm Test Code for the lab that services your account.
In-home collection is not available in your area through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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