Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative, Real-Time PCR

Test Code

34257

CPT Code(s)

87529 (x2)

Test Code

34257

CPT Code(s)

87529 (x2)

Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Herpes Simplex Virus, Type 1 and 2 DNA, Qualitative, Real-Time PCR - DNA testing is analytically more sensitive than culture, especially in patients with encephalitis or meningitis. DNA testing may be useful in diagnosis of infection in neonates. Neonates who have been exposed to HSV can develop disseminated infection and encephalitis. Encephalitis is usually due to HSV I whereas meningitis is usually due to HSV II. DNA testing provides reliable means to define the type.

Test Resources

Clinical Focus

Genital Herpes: Laboratory Support of Diagnosis and Management

Test Details

Methodology

Real-Time Polymerase Chain Reaction

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by the U.S. Food and Drug Administration. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

HSV 1 DNA	Not detected
HSV 2 DNA	Not detected

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

Real-Time Polymerase Chain Reaction

Assay Category

Reference Range(s)

HSV 1 DNA	Not detected
HSV 2 DNA	Not detected

LOINC® Codes, Performing Laboratory

Service Area must be determined

Alternative Specimen(s)

Bronchoalveolar lavage or bronchial wash

Collection Instructions

CSF: Collect in a sterile leak-proof container and store refrigerated or frozen.
Swab: Swab a lesion and place swab in viral transport media, VCM, M4, or Aptima® Unisex Collection device (white label)
Serum: Collect blood in serum separator tubes (SST). Transfer serum into sterile leak-proof container and store refrigerated or frozen.
Bronchial wash and BAL: Collect in a sterile leak-proof container.

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

⁠⁠⁠⁠⁠⁠⁠Aptima® Unisex Collection device (white label)
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days

All other container types
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)

Setup Schedule, Preferred Specimen(s), Minimum Volume

Service Area must be determined

Alternative Specimen(s)

Bronchoalveolar lavage or bronchial wash

Collection Instructions

Transport Temperature

Refrigerated (cold packs)

Specimen Stability

⁠⁠⁠⁠⁠⁠⁠Aptima® Unisex Collection device (white label)
Room temperature: 14 days
Refrigerated: 14 days
Frozen: 30 days

All other container types
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 30 days (whole blood unacceptable)

Setup Schedule, Preferred Specimen(s), Minimum Volume

Service Area must be determined

Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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