Epstein Barr Virus DNA, Qualitative, Real-Time PCR
Test Code
Clinical Significance
Epstein Barr Virus DNA, Qualitative, Real-Time PCR - Infection with EBV is common and is generally subclinical or presents as a self-limited illness. Reactivation of latent EBV in an immunocompromised person can lead to more serious results, including lymphoproliferative disorders or neurological disease. PCR methods may be useful in identifying EBV in a variety of clinical specimens.
Test Resources
Test Details
Methodology
Reference Range(s)
Alternative Name(s)
Lymphoproliferative
,Infectious MononucleosisPreferred Specimen(s)
1 mL whole blood collected in an EDTA (lavender-top) tube or
1 mL plasma collected in an EDTA (lavender-top) tube or
1 mL serum or
1 mL CSF collected in a sterile plastic leak-proof container or
>3 cubic mm fresh tissue collected in a sterile plastic leak-proof container
Alternative Specimen(s)
1 mL amniotic fluid or lower respiratory specimen (BAL, bronchial wash, tracheal secretions) collected in a sterile plastic leak-proof container • 0.3 undiluted vitreous (eye) fluid collected in a sterile plastic leak-proof container
Minimum Volume
3 cubic mm tissue • 0.25 mL undiluted vitreous (eye) fluid • 0.3 mL all other fluids
Collection Instructions
CSF: Submit in a sterile plastic container and store refrigerated or frozen.
Tissue: Submit in a sterile plastic container and transport frozen.
Raw vitreous (eye) fluid specimens: Do not dilute collected specimen with additional saline or other transport media.
Do not freeze whole blood.
Transport Container
EDTA (lavender-top) tube
Transport Temperature
Tissue: Frozen
All other samples: Refrigerated (cold packs)
Specimen Stability
- Whole blood
- Room temperature: 48 hours
- Refrigerated: 8 days
- Frozen: Unacceptable
- All other specimens
- Room temperature: 48 hours
- Refrigerated: 8 days
- Frozen: 30 days
Reject Criteria
Hemolyzed whole blood • Heparin whole blood
Setup Schedule
1 mL plasma collected in an EDTA (lavender-top) tube or
1 mL serum or
1 mL CSF collected in a sterile plastic leak-proof container or
>3 cubic mm fresh tissue collected in a sterile plastic leak-proof container
Tissue: Submit in a sterile plastic container and transport frozen.
Raw vitreous (eye) fluid specimens: Do not dilute collected specimen with additional saline or other transport media.
Do not freeze whole blood.
All other samples: Refrigerated (cold packs)
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: Unacceptable
All other specimens
Room temperature: 48 hours
Refrigerated: 8 days
Frozen: 30 days