Pregnenolone, LC/MS
Test Code
Clinical Significance
Pregnenolone, LC/MS - This test may be useful in differentiating among different types of congenital adrenal hyperplasia (CAH). This test may also be used to monitor progress and treatment after diagnosis [1].
CAH is a group of autosomal recessive diseases characterized by deficiencies of enzymes in steroid hormone production. These deficiencies cause imbalances of steroid intermediates and hormones. Clinical manifestations of CAH vary and depend upon the type of defect and severity of the impairment. Therefore, measurement of different steroid hormones can help in diagnosing CAH [1,2].
Pregnenolone is ...
Test Resources
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Test Details
Methodology
Reference Range(s)
| Adult | 22-237 ng/dL |
| Pediatric | |
| 1-59 days | 68-1303 ng/dL |
| 60 days-1 year | ≤219 ng/dL |
| 2-6 years | ≤140 ng/dL |
| 7-9 years | ≤156 ng/dL |
| 10-12 years | 15-220 ng/dL |
| 13-17 years | 12-196 ng/dL |
Preferred Specimen(s)
0.5 mL serum collected in a red-top tube (no gel)
Minimum Volume
0.2 mL
Collection Instructions
Collect blood in a Vacutainer® with no additives. Allow blood to clot (30 minutes) at room temperature, 18-25° C. Centrifuge and separate the serum from the cells. Freeze as soon as possible.
Transport Container
Transport tube
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
- Refrigerated: 72 hours
- Frozen -20° C: 14 days
- Frozen -70° C: 30 days
Reject Criteria
Gross hemolysis • Serum separator tube (SST)
Setup Schedule
Refrigerated: 72 hours
Frozen -20° C: 14 days
Frozen -70° C: 30 days