Testosterone, Free, Bioavailable and Total, Males (Adult), Immunoassay

Testosterone, Free, Bioavailable and Total, Males (Adult), Immunoassay

Test Code

30741
82040, 84270, 84403
30741
82040, 84270, 84403
Not offered in Quest Nichols Institute - Chantilly, VA | Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano | Quest Nichols Institute -San Juan Capistrano, CA. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Testosterone, Free, Bioavailable and Total, Males (Adult), Immunoassay -

This test panel may be helpful in assessing androgen status and screening for hypogonadism in men. This immunoassay cannot accurately measure the low testosterone levels generally found in women, children, and men with hypogonadism [1].

Testosterone circulates in 3 major forms: unbound (free), weakly bound to albumin, and tightly bound to sex hormone-binding globulin (SHBG). Free testosterone comprises 2% to 4% of total testosterone and is biologically active. Albumin-bound testosterone can dissociate from albumin in capillaries and become available to tissue. Thus, bioavailable testosterone ...

Test Resources

Test FAQ

Hypogonadism

Test FAQ

Testosterone Testing

Clinical Focus

Hypogonadism and Low Testosterone in Men: Laboratory Support of Diagnosis and Management

Test Details

Includes

  • Testosterone, Total, Males (Adult), Immunoassay
  • Albumin
  • Sex Hormone Binding Globulin (SHBG)
  • Testosterone, Free and Bioavailable (calculated)

Patient Preparation

Testosterone, Total, Males (Adult), Immunoassay: Due to changes in testosterone levels throughout the day, two morning (8:00-10:00 a.m.) specimens obtained on different days are recommended by The Endocrine Society for screening.

Reference Range(s)

Testosterone, Total, Males (Adult), Immunoassay

Male 250-827 ng/dL
Female Not applicable
All test requests for Testosterone on female and pediatric (<18 years) patients must use test code 15983 - Testosterone, Total, MS.

Albumin 3.6-5.1 g/dL

Sex Hormone Binding Globulin
Age Male (nmol/L) Female (nmol/L)
<3 Years Not established Not established
3-9 Years 32-158 32-158
10-13 Years 20-166 24-120
14-17 Years 20-87 12-150
18-55 Years 10-50 17-124
>55 Years 22-77 14-73

Free Testosterone
Age Male (pg/mL) Female
18-69 Years 46.0-224.0 Not applicable
>69 Years 6.0-73.0 Not applicable
Bioavailable Testosterone
Age Male (ng/dL) Female
18-69 Years 110.0-575.0 Not applicable
>69 Years 15.0-150.0 Not applicable

LOINC® Codes, Performing Laboratory

Service Area must be determined
Testosterone, Total, Males (Adult), Immunoassay
Albumin
Sex Hormone Binding Globulin (SHBG)
Testosterone, Free and Bioavailable (calculated)
Testosterone, Total, Males (Adult), Immunoassay: Due to changes in testosterone levels throughout the day, two morning (8:00-10:00 a.m.) specimens obtained on different days are recommended by The Endocrine Society for screening.

Reference Range(s)

Testosterone, Total, Males (Adult), Immunoassay
Male 250-827 ng/dL
Female Not applicable
All test requests for Testosterone on female and pediatric (<18 years) patients must use test code 15983 - Testosterone, Total, MS.

Albumin 3.6-5.1 g/dL

Sex Hormone Binding Globulin
Age Male (nmol/L) Female (nmol/L)
<3 Years Not established Not established
3-9 Years 32-158 32-158
10-13 Years 20-166 24-120
14-17 Years 20-87 12-150
18-55 Years 10-50 17-124
>55 Years 22-77 14-73

Free Testosterone
Age Male (pg/mL) Female
18-69 Years 46.0-224.0 Not applicable
>69 Years 6.0-73.0 Not applicable
Bioavailable Testosterone
Age Male (ng/dL) Female
18-69 Years 110.0-575.0 Not applicable
>69 Years 15.0-150.0 Not applicable
Service Area must be determined

Preferred Specimen(s)

2 mL serum collected in a red-top tube (no gel)

Alternative Specimen(s)

Serum separator tube (SST)

Minimum Volume

1.5 mL

Collection Instructions

Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation.

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • See individual tests

Reject Criteria

See individual tests

Setup Schedule

Service Area must be determined
2 mL serum collected in a red-top tube (no gel)
Serum separator tube (SST)
1.5 mL
Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation.
Transport tube
Room temperature
See individual tests
See individual tests
Service Area must be determined
Not offered in Quest Nichols Institute - Chantilly, VA | Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano | Quest Nichols Institute -San Juan Capistrano, CA. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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