Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Test Code
Clinical Significance
Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM) - Beta-2-Glycoprotein 1 (also known as apolipoprotein H), is a cofactor in antiphospholipid antibody binding and is the critical antigen in the antiphospholipid antibody syndrome. The presence of high titer IgG/IgM beta-2-glycoprotein 1 antibodies is one criteria for the diagnosis of antiphospholipid antibody syndrome.
Test Resources
Test FAQ
Antiphospholipid AntibodiesClinical Focus
Autoimmune Rheumatic and Related DiseasesTest Details
Methodology
Reference Range(s)
| B2-Glycoprotein I (IgG) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
| B2-Glycoprotein I (IgA) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
| B2-Glycoprotein I (IgM) Ab | <20 U/mL Antibody not detected ≥20 U/mL Antibody detected |
Preferred Specimen(s)
3 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube
Alternative Specimen(s)
Serum
Minimum Volume
1.5 mL
Collection Instructions
Plasma: Centrifuge light blue-top tube 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
Room temperature: 5 days
- Refrigerated: 14 days
- Frozen: 30 days
Reject Criteria
Gross hemolysis • Grossly lipemic
Setup Schedule
3 mL plasma collected in a 3.2% sodium citrate (light blue-top) tube
Room temperature
Room temperature: 5 days
Refrigerated: 14 days
Frozen: 30 days