ANA Screen,IFA, with Reflex to Titer and Pattern

ANA Screen,IFA, with Reflex to Titer and Pattern

Test Code

249
86038
249
86038
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

ANA Screen,IFA, with Reflex to Titer and Pattern -

This immunofluorescence assay (IFA) is often ordered as part of an initial diagnostic evaluation of individuals with clinical suspicion of autoimmune diseases associated with antinuclear antibodies (ANAs). The American College of Rheumatology (ACR) recommends IFA on human epithelial type 2 (HEp-2) cells as the gold standard method for ANA testing because of its overall high sensitivity [1].

ANAs are associated with several autoimmune diseases, such as systemic lupus erythematosus, systemic sclerosis, mixed connective tissue disease, polymyositis, primary biliary cholangitis, rheumatoid ...

Test Resources

Test FAQ

Tests for Autoimmune Diseases (ANA)

Algorithm

ANA, IFA, Cascade with Rheumatoid Arthritis Panel 2, with Reflexes (test code 94954).

Clinical Focus

Autoimmune Rheumatic and Related Diseases

Clinical Focus

Systemic Sclerosis: Laboratory Markers for Diagnosis and Prognosis

Topic Brief

Laboratory Diagnosis of Peripheral Neuropathy

Test Summary

ANA, IFA, Cascade and Rheumatoid Arthritis Panel 2, With Reflexes

Test Summary

ANAlyzeR ANA, IFA with Reflex Titer/Pattern, Systemic Autoimmune Panel 1

Test Details

Includes

  • If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Methodology

Immunofluorescence Assay (IFA)

Reference Range(s)

ANA Screen Negative
  ANA Titer  
  <1:40 Negative
  1:40-1:80 Low antibody level
  >1:80 Elevated antibody level

Alternative Name(s)

FANA,Antinuclear Antibody Screen,Fluorescent ANA,Hep-2,Progressive ANA

LOINC® Codes, Performing Laboratory

Service Area must be determined
If ANA Screen, IFA is positive, then ANA Titer and Pattern will be performed at an additional charge (CPT code(s): 86039).

Methodology

Immunofluorescence Assay (IFA)

Reference Range(s)

ANA Screen Negative
  ANA Titer  
  <1:40 Negative
  1:40-1:80 Low antibody level
  >1:80 Elevated antibody level

Alternative Name(s)

FANA,Antinuclear Antibody Screen,Fluorescent ANA,Hep-2,Progressive ANA
Service Area must be determined

Preferred Specimen(s)

1 mL serum

Minimum Volume

0.5 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

  • Room temperature: 4 days
  • Refrigerated: 7 days
  • Frozen: 30 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Microbial contamination may interfere

Setup Schedule

Service Area must be determined
1 mL serum
0.5 mL
Transport tube
Room temperature
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 30 days
Gross hemolysis • Grossly lipemic • Microbial contamination may interfere
Service Area must be determined
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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