Activated Protein C-Resistance

Activated Protein C-Resistance

Test Code

22
85307
22
85307
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection is not available in your area through Quest Mobile.

Clinical Significance

Activated Protein C-Resistance - Activated protein C (APC) resistance is the most frequent hereditary defect associated with deep vein thrombosis. Over 95% of the APC resistance phenotype is due to the Factor V Leiden mutation. The APC-Resistance assay is a plasma based functional test for the determination of APC resistance caused by the factor V Leiden mutation.

Test Resources

None found for this test
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Test Details

Methodology

RVVT-based Assay (Clot-based)

Reference Range(s)

≥2.1 Ratio

Alternative Name(s)

APC Resistance,APCR

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

RVVT-based Assay (Clot-based)

Reference Range(s)

≥2.1 Ratio

Alternative Name(s)

APC Resistance,APCR
Service Area must be determined

Preferred Specimen(s)

2 mL frozen platelet-poor citrated plasma collected in a 3.2% sodium citrate (light-blue-top) tube

Minimum Volume

1 mL

Collection Instructions

Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.

Transport Container

Transport tube

Transport Temperature

Frozen

Specimen Stability

  • Room temperature: Unacceptable
  • Refrigerated: Unacceptable
  • Frozen: 60 days

Reject Criteria

Gross hemolysis • Clotted specimen • Serum

Setup Schedule

Service Area must be determined
2 mL frozen platelet-poor citrated plasma collected in a 3.2% sodium citrate (light-blue-top) tube
1 mL
Platelet-poor plasma: Centrifuge light blue-top tube for 15 minutes at approximately 1500 g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial. Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL). Freeze immediately and ship on dry ice.
Transport tube
Frozen
Room temperature: Unacceptable
Refrigerated: Unacceptable
Frozen: 60 days
Gross hemolysis • Clotted specimen • Serum
Service Area must be determined
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection is not available in your area through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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