c-KIT Mutation Analysis, Cell Based

c-KIT Mutation Analysis, Cell Based

Test Code

19961
81272
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
19961
81272
CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

c-KIT Mutation Analysis, Cell Based - Activating c-KIT mutations have been indentified in various human cancers. c-KIT exon 8 and 17 mutations have been described in patients with CBF-AMLs and usually confer a poor prognosis with increased relapse rate. c-KIT exon 9, 11, 13, 17 mutations have been reported in nearly 90% GIST patients. The presence mutation usually predict poor survival. c-KIT exon 17 mutation has been reported in patients with systemic mastocytosis.

Test Resources

Clinical Focus

Cutaneous Melanoma: Laboratory Support of Diagnosis and Management

Test Details

Methodology

Next Generation Sequencing

Reference Range(s)

Not detected

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

Next Generation Sequencing

Reference Range(s)

Not detected
Service Area must be determined

Preferred Specimen(s)

3 mL whole blood collected in an EDTA (lavender-top) tube or formalin fixed paraffin embedded tissue

Alternative Specimen(s)

Bone marrow (refrigerated preferred transport) • Cell pellet

Minimum Volume

1 mL

Collection Instructions

Whole blood (preferred): Follow standard whole blood collection procedure. Collect 3-5 mL whole blood samples in EDTA tube. Blood samples are shipped at room temperature or 4° C. Do not freeze whole blood. Record the draw time and date on the tube. Ship immediately to maintain sample stability.
Cell pellet: This assay is also designed for the remnant cell pellet from Cytogenetics. The cell pellet usually fixed should be transported at room temperature or refrigerated to the laboratory as soon as possible. Please provide a copy of the Cytogenetics report.

Transport Container

EDTA (lavender-top) tube or formalin fixed paraffin embedded tissue block

Transport Temperature

Room temperature

Specimen Stability

  • Whole blood and bone marrow
  • Room temperature: 7 days
  • Refrigerated: 14 days
  • Frozen: Unacceptable
  •  
  • Tissue
  • Room temperature: Indefinite
  • Refrigerated: Indefinite
  • Frozen: Do not freeze

Setup Schedule, Reject Criteria

Service Area must be determined
3 mL whole blood collected in an EDTA (lavender-top) tube or formalin fixed paraffin embedded tissue
Bone marrow (refrigerated preferred transport) • Cell pellet
1 mL
Whole blood (preferred): Follow standard whole blood collection procedure. Collect 3-5 mL whole blood samples in EDTA tube. Blood samples are shipped at room temperature or 4° C. Do not freeze whole blood. Record the draw time and date on the tube. Ship immediately to maintain sample stability.
Cell pellet: This assay is also designed for the remnant cell pellet from Cytogenetics. The cell pellet usually fixed should be transported at room temperature or refrigerated to the laboratory as soon as possible. Please provide a copy of the Cytogenetics report.
EDTA (lavender-top) tube or formalin fixed paraffin embedded tissue block
Room temperature
Whole blood and bone marrow
Room temperature: 7 days
Refrigerated: 14 days
Frozen: Unacceptable

Tissue
Room temperature: Indefinite
Refrigerated: Indefinite
Frozen: Do not freeze
Service Area must be determined
Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.
In-home collection may be available in your area for purchase through Quest Mobile.
Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

The tests listed by specialty and category are a select group of tests offered. For a complete list of Quest Diagnostics tests, please adjust the filter options chosen, or refer to our Directory of Services.

Customize the test information for your Service Area