ANA Multiplex with Reflex to 11 Antibody Cascade
Test Code
Clinical Significance
ANA Multiplex with Reflex to 11 Antibody Cascade - This panel can be helpful in the diagnosis of autoimmune rheumatic diseases, including systemic lupus erythematosus, systemic sclerosis, Sjogren syndrome, mixed connective tissue disease, and rheumatoid arthritis. All specimens are screened for antinuclear antibodies (ANAs) with multiplex immunoassay. This approach simultaneously detects antibodies to dsDNA, chromatin, ribosomal protein, SSA, SSB, Smith (Sm), Sm/RNP, RNP, Scl-70, Jo-1, and centromere B. A positive result indicates the presence of at least 1 of these antibodies; a negative result indicates that none of the component antibodies were ...
Test Resources
Test FAQ
Tests for Autoimmune Diseases (ANA)Clinical Focus
Autoimmune Rheumatic and Related DiseasesTest Details
If the ANA Screen, Immunoassay is positive, it will reflex the following five antibodies at an additional charge: dsDNA (CPT code(s): 86225), Sm/RNP (CPT code(s): 86235), RNP (CPT code(s): 86235), Sm (CPT code(s): 86235), and Chromatin (CPT code(s): 86235).
If any of those five antibodies are positive, the cascade stops and the results are reported.
If all five of those antibodies are negative, four additional antibodies will be reflexed at an additional charge: SSA (CPT code(s): 86235), SSB (CPT code(s): 86235), Scl-70 (CPT code(s): 86235), Jo-1 (CPT code(s): 86235).
If any of those four antibodies are positive, the cascade stops and the results are reported.
If all four of those antibodies are negative, the following two additional antibodies will be reflexed at an additional charge: Ribosomal P (CPT code(s): 83516) and Centromere B (CPT code(s): 86235).
The cascade stops upon the first positive antibody found in a group. It is possible that antibodies in subsequent groups are also positive, but will not be added or reported. Please contact your local Quest Diagnostics Laboratory if you are interested in adding this additional testing.
Methodology
Reference Range(s)
| ANA Screen, Immunoassay | Negative |
Alternative Name(s)
Preferred Specimen(s)
1 mL serum
Minimum Volume
0.5 mL
Transport Container
Transport tube
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 30 days
Reject Criteria
Gross hemolysis • Grossly lipemic • Grossly icteric
Setup Schedule
Refrigerated: 7 days
Frozen: 30 days