Immunoassay (IA)
Specimen requirements | |
|---|---|
| Patient preparation | A minimum of 12 hours fasting is required. Fasting morning collection 8-10 am (Diurnal variations cause elevated levels at night). Dietary supplements containing biotin may interfere in assays and may skew results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. |
| Preferred specimen(s) | 1 mL serum |
| Minimum volume | 0.5 mL |
| Collection instructions | Allow blood to clot (30-60 minutes) at room temperature. Centrifuge and separate the serum from the cells and transfer serum to a clean transport tube. Freeze as soon as possible. |
| Transport container | Transport tube |
| Transport temperature | Frozen |
| Specimen stability | Room temperature: 16 hours Refrigerated: 72 hours Frozen: 90 days |
| Reject criteria | Moderate to gross hemolysis • Grossly lipemic • Gross icteric |
| Adult Male | |||||||||||||
| 18-29 Years | 87-1200 pg/mL | ||||||||||||
| 30-39 Years | 70-780 pg/mL | ||||||||||||
| 40-49 Years | 60-700 pg/mL | ||||||||||||
| 50-68 Years | 87-345 pg/mL | ||||||||||||
| Adult Female | |||||||||||||
| 18-29 Years | 60-640 pg/mL | ||||||||||||
| 30-39 Years | 60-650 pg/mL | ||||||||||||
| 40-49 Years | 50-465 pg/mL | ||||||||||||
| >49 Years | Not established | ||||||||||||
| Pediatric Male | |||||||||||||
| 5-9 Years | 574-1849 pg/mL | ||||||||||||
| 10-13 Years | 519-2415 pg/mL | ||||||||||||
| 14-17 Years | 435-2924 pg/mL | ||||||||||||
| Pediatric Female | |||||||||||||
| 5-9 Years | 574-1849 pg/mL | ||||||||||||
| 10-13 Years | 519-2415 pg/mL | ||||||||||||
| 14-17 Years | 242-1291 pg/mL | ||||||||||||
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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