Mycoplasma pneumoniae DNA, Qualitative, Real-Time PCR

Test code

15498

CPT code(s)*

87581

**CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

Please provide Service area information to confirm Test Code for the lab that services your account.

In-home collection is not available in your area through Quest Mobile.

Clinical significance

Mycoplasma pneumoniae DNA, Qualitative, Real-Time PCR - Mycoplasma pneumoniae DNA, PCR, is a highly sensitive method to detect the presence of Mycoplasma pneumoniae DNA in clinical specimens. The diagnosis of Mycoplasma pneumoniae infection should not rely solely upon the result of a PCR assay. A positive result should be determined in conjunction with clinical presentation and additional established clinical tests. Moreover, this assay cannot differentiate between viable and nonviable organisms. A negative PCR result indicates the absence of Mycoplasma pneumoniae DNA at detectable levels in the sample tested and does not exclude the diagnosis of…

Test resources

None found for this test
Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities.

Details

Test details

Methodology

Real-Time Polymerase Chain Reaction

Assay category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Alternative name(s)

M. pneumoniae by PCR

Specimen requirements
Patient preparation	None Specified
Preferred specimen(s)	1 mL sputum or bronchial lavage/wash collected in a sterile, leak-proof plastic container or throat or nasopharyngeal swab in M4 media or V-C-M medium (green-cap) tube or equivalent (UTM)
Alternative specimen(s)	Cerebrospinal fluid
Minimum volume	0.35 mL sputum or bronchial lavage/wash
Collection instructions	Nasopharyngeal and throat swab in M4, V-C-M (UTM) media: Use only sterile Dacron® or rayon swabs. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing. Break applicator sticks off near the tip to permit tightening of the cap. Sputum: Collect in a sputum collection kit or a sterile, leak-proof plastic container. Bronchial lavage: Collect in a sterile, leak-proof plastic container.
Transport container	Sterile leak-proof plastic container
Transport temperature	Refrigerated (cold packs)
Specimen stability	Room temperature: 48 hours Refrigerated: 7 days Frozen: 30 days
Reject criteria	Calcium alginate swabs

Reference range(s)

Not detected

LOINC® codes

Select a service areato view this section

Service Area Information

Performing lab & setup schedule

Select a service areato view this section

Service Area Information

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

The tests listed by specialty and category are a select group of tests offered. For a complete list of Quest Diagnostics tests, please adjust the filter options chosen, or refer to our Directory of Services.

Customize the test information for your service area