Cyclosporine A Trough, LC/MS/MS, Blood

Test Code

15220

CPT Code(s)

80158

Test Code

15220

CPT Code(s)

80158

Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection is not available in your area through Quest Mobile.

Clinical Significance

Cyclosporine A Trough, LC/MS/MS, Blood - Cyclosporine is a commonly used immunosuppressive drug in patients receiving transplants. LC/MS/MS methods have higher specificity for the parent compound than immunoassay. Therapeutic drug monitoring is useful to optimize dose and avoid toxicity. High cyclosporine levels can lead to nephrotoxicity; Low levels can lead to organ rejection following transplant. Peak concentrations are reached at around 3.5 hours after oral dosage. Elimination half-life is 10-27 hours.

Test Resources

Topic Brief

Drug Half-Life, Steady State, and Recommended Sample Collection Time

Test Guide

Solid Organ and Hematopoietic Stem Cell Transplantation Laboratory Testing for Donors and Recipients

Test Details

Patient Preparation

Optimum time to collect sample is 1 hour before next dose

Methodology

Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

No definitive therapeutic or toxic ranges have been established. Opitmal blood drug levels are influenced by type of transplant, patient response, time post-transplant, co-administration of other drugs, and drug formulation.

The following trough ranges are suggested guidelines:

Kidney Transplantation	100-200 mcg/L
Other Organ Transplant	200-300 mcg/L

LOINC® Codes, Performing Laboratory

Service Area must be determined

Patient Preparation

Optimum time to collect sample is 1 hour before next dose

Methodology

Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)

Assay Category

Reference Range(s)

Kidney Transplantation	100-200 mcg/L
Other Organ Transplant	200-300 mcg/L

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

5 mL whole blood collected in an EDTA (lavender-top) tube

Alternative Specimen(s)

Whole blood collected in: Sodium heparin (green-top) tube

Minimum Volume

2 mL

Transport Container

EDTA (lavender-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 6 days
Frozen: Unacceptable

Reject Criteria

Clotted specimen

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

5 mL whole blood collected in an EDTA (lavender-top) tube

Alternative Specimen(s)

Whole blood collected in: Sodium heparin (green-top) tube

Minimum Volume

2 mL

Transport Container

EDTA (lavender-top) tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 7 days
Refrigerated: 6 days
Frozen: Unacceptable

Reject Criteria

Clotted specimen

Setup Schedule

Service Area must be determined

Ordering Restrictions may apply. Please provide SERVICE AREA INFORMATION to find available tests you can order.

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection is not available in your area through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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