Antiphospholipid Antibody Panel
Test Code
Clinical Significance
Antiphospholipid Antibody Panel - The antiphospholipid antibody syndrome (APS) is a clinical-pathologic correlation that includes a clinical event (e.g. arterial or venous thrombosis, pregnancy morbidity) and persistent positive antiphospholipid antibodies (IgM, IgG Cardiolipin or b2GPI antibodies greater than the 99th percentile; or a lupus anticoagulant). International consensus guidelines for APS suggest waiting at least 12 weeks before retesting to confirm antibody persistence. The Systemic Lupus International Collaborating Clinics immunological classification criteria for systemic lupus erythematosus (SLE) include testing ...
Test Resources
Clinical Focus
Autoimmune Rheumatic and Related DiseasesTest Details
Phosphatidylserine/Prothrombin (PS/PT) Antibodies (IgG, IgM)
Cardiolipin Antibodies (IgA, IgG, IgM)
Methodology
Reference Range(s)
Alternative Name(s)
Preferred Specimen(s)
1 mL plasma collected in each of two 3.2% sodium citrate (light blue-top) tubes
Alternative Specimen(s)
Serum collected in each of two separate tubes
Minimum Volume
0.5 mL each specimen
Collection Instructions
Plasma (preferred): Centrifuge light blue-top tube 15 minutes at approximately 1500 x g within 60 minutes of collection. Using a plastic pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer and place into a plastic vial.
Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Serum: Centrifuge a red-top tube (no gel) or serum separator tube 15 minutes at approximately 1500 g within 24 hours of collection. Transfer serum into a plastic specimen vial.
Transport Container
Transport tube(s)
Transport Temperature
Room temperature
Specimen Stability
- Room temperature: 5 days
Refrigerated: 14 days
Frozen: 30 days
Reject Criteria
Gross hemolysis • Grossly lipemic
Setup Schedule
Centrifuge a second time and transfer platelet-poor plasma into a new plastic vial. Plasma must be free of platelets (<10,000/mcL).
Serum: Centrifuge a red-top tube (no gel) or serum separator tube 15 minutes at approximately 1500 g within 24 hours of collection. Transfer serum into a plastic specimen vial.
Room temperature
Refrigerated: 14 days
Frozen: 30 days