HIV-1 and HIV-2 RNA, Qualitative Real-Time PCR

Test code

14312

CPT code(s)*

87535, 87538

**CPT Code is subject to a Medicare Limited Coverage Policy and may require a signed ABN when ordering.

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical significance

HIV-1 and HIV-2 RNA, Qualitative Real-Time PCR - This test is an in vitro nucleic acid amplification test (NAAT) for the qualitative detection of human immunodeficiency virus (HIV) types 1 and 2 RNA in human serum and plasma. The test is intended to be used as an aid in diagnosis of HIV-1 and 2 infections. In individuals without antibodies to HIV-1 or HIV-2, the presence of HIV-1 or 2 nucleic acid in the plasma or serum is indicative of acute infection. The HIV-1 and 2 Qualitative PCR may be used as part of a multi-step diagnostic algorithm to confirm the presence of HIV-1 or 2 infection in specimens reactive for HIV-1 and 2 antibodies or HIV antigen [1]. For infants with perinatal HIV…

Test resources

None found for this test
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Details

Test details

Methodology

Real-Time Polymerase Chain Reaction

Specimen requirements
Patient preparation	None Specified
Preferred specimen(s)	1.6 mL serum
Alternative specimen(s)	Plasma collected in: EDTA (lavender-top) tube
Minimum volume	0.8 mL
Collection instructions	Serum: Collect in a serum separator tube or red-top tube (no gel). The tube should be centrifuged after clotting. Spun serum separator tubes may be submitted at room temperature or refrigerated unopened without transfer to a plastic screw-cap vial. Red-top tube (no gel) serum should be transferred to a plastic screw-cap vial and submitted for testing. Plasma: Collect in an EDTA (lavender-top) tube and centrifuge tube within 24 hours of collection. Transfer plasma to a plastic screw-cap vial, label as plasma and submit for testing. Submitted tubes must be labeled with patient identifier and submitted only for HIV testing.
Transport container	Serum separator tube (SST)
Transport temperature	Room temperature
Specimen stability	Room temperature: 72 hours Refrigerated: 5 days Frozen: 42 days
Reject criteria	Frozen specimens submitted in original collection container

Reference range(s)

Reference Range(s)

HIV 1 RNA, QL RT PCR	Not Detected
HIV 2 RNA, QL RT PCR	Not Detected

LOINC® codes

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Service Area Information

Performing lab & setup schedule

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Service Area Information

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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