Mpox Virus DNA, Qualitative Real-Time PCR
Test Code
Clinical Significance
Mpox Virus DNA, Qualitative Real-Time PCR - The Mpox Virus DNA, Qualitative Real-time PCR test is intended for the qualitative detection of non-variola Orthopoxviruses and Mpox virus (clade II) DNA using swabs from human pustular or vesicular rash specimens.
Test Resources
Specimen Handling - Specimen Collection Guide
Monkeypox Specimen Collection GuideTest Details
Methodology
Reference Range(s)
| Orthopoxvirus DNA, QL PCR | Not detected |
| Mpox Virus DNA, QL PCR | Not detected |
Alternative Name(s)
Preferred Specimen(s)
Lesion swab collected in 3 mL viral culture media (VCM) or equivalent
Minimum Volume
0.5 mL
Collection Instructions
Swab a pustule/lesion vigorously and place the swab into a viral culture media (or equivalent) tube.
No additional confirmatory testing is required at the CDC, therefore a duplicate swab from the same lesion is not needed. If clinically indicated, consider submitting additional swabs if multiple lesions with different stages are present. Multiple specimens collected on a single patient should be submitted separately and be accompanied by its own separate requisition and transported in its own sealed bag.
Transport Container
VCM, VTM, UTM or equivalent
Transport Temperature
Frozen
Specimen Stability
- Room temperature: Unacceptable
Refrigerated: 7 days
Frozen: 30 days
Reject Criteria
Calcium Alginate swabs • Cotton swabs • Wooden shaft swabs • Dry swabs (e.g., not submitted in VCM or equivalent)
Setup Schedule
No additional confirmatory testing is required at the CDC, therefore a duplicate swab from the same lesion is not needed. If clinically indicated, consider submitting additional swabs if multiple lesions with different stages are present. Multiple specimens collected on a single patient should be submitted separately and be accompanied by its own separate requisition and transported in its own sealed bag.
Refrigerated: 7 days
Frozen: 30 days