Culture, Select Agent
Test Code
Clinical Significance
Culture, Select Agent - Pathogens recovered with this culture fit the categorical description of a Select Agent defined by the CDC and NIH. These pathogens include but are not limited to Bacillus anthracis, Brucella, Francisella tularensis, Yersinia pestis, Burkholderia mallei, Burkholderia pseudomallei. These select agents may cause anthrax, plague, mellioidosis and glanders, tularemia, and brucellosis. Each of these can cause septicemia, pneumonia, and tissue involvement.
Test Resources
Test Details
Methodology
Includes routine isolation procedures. Suspicious isolates referred to state/local health department for definitive identification. Susceptibility testing not performed.
Reference Range(s)
Alternative Name(s)
Preferred Specimen(s)
5 mL or 5 grams abscess collected in a Amies liquid transport swab, Amies gel transport swab, Amies liquid elution swab (ESwab) or equivalent or
5 mL or 5 grams tissue collected in a sterile leak-proof container
Alternative Specimen(s)
Collected in a sterile leak-proof container: Sputum, bronchial washing/lavage, or vesicular fluid/aspirate • Cutaneous lesion collected in: Amies liquid transport swab, Amies gel transport swab, Amies liquid elution swab (ESwab) or equivalent • BacT/ALERT blood culture bottle set: Blood or bone marrow
Minimum Volume
1 mL • 1 gram
Collection Instructions
Indicate suspected organism
Transport Container
Amies liquid transport swab, Amies gel transport swab
Amies liquid elution swab (ESwab) or equivalent
Sterile leak-proof container
Transport Temperature
Swabs, blood or bone marrow: Room temperature
Tissue, sputum, bronchial washing/lavage, vesicular fluid/ aspirate: Refrigerated (cold packs)
Specimen Stability
Abscess or cutaneous lesion swab, tissue, or vesicular fluid/aspirate
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: UnacceptableRespiratory
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: UnacceptableBlood and bone marrow
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Reject Criteria
Viral transport medium • DNA Probe transport device • Dry swabs • Reject the following and immediately notify your supervisor, manager, or director, who will notify the appropriate public health authority: Environmental specimens, Suspicious substances (powders), or Veterinary specimens
Setup Schedule
5 mL or 5 grams tissue collected in a sterile leak-proof container
Amies liquid elution swab (ESwab) or equivalent
Sterile leak-proof container
Tissue, sputum, bronchial washing/lavage, vesicular fluid/ aspirate: Refrigerated (cold packs)
Abscess or cutaneous lesion swab, tissue, or vesicular fluid/aspirate
Room temperature: 48 hours
Refrigerated: 48 hours
Frozen: Unacceptable
Respiratory
Room temperature: Unacceptable
Refrigerated: 48 hours
Frozen: Unacceptable
Blood and bone marrow
Room temperature: 48 hours
Refrigerated: Unacceptable
Frozen: Unacceptable