Bordetella pertussis/parapertussis DNA, Qualitative, Real-Time PCR
Test code
CPT code(s)*
Test resources
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Details
Includes
Bordetella pertussis DNA and Bordetella parapertussis DNA
Methodology
Real-Time Polymerase Chain Reaction (PCR)
This test detects but does not distinguish between B. Pertussis and B. Holmesii.
Assay category
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Alternative name(s)
Whooping Cough
Specimen requirements | |
|---|---|
| Patient preparation | None Specified |
| Preferred specimen(s) | Nasopharyngeal aspirate or nasopharyngeal swab |
| Alternative specimen(s) | Nasopharyngeal swab submitted in a VCM medium (green-cap) tube or equivalent UTM • Multi Microbe Media (M4) • eSwabs |
| Minimum volume | Select a service area to view this section. |
| Collection instructions | Nasopharyngeal aspirates: Instill 1-1.5 mL of nonbacteriostatic saline (pH 7.0) into one nostril. Flush a plastic catheter or tubing with 2-3 mL of saline. Insert the tubing into the nostril parallel to the palate. Aspirate nasopharyngeal secretions. Repeat this procedure for the other nostril. Combine aspirates into a sterile vial. Nasopharyngeal swab: Collection kits using Amies liquid, M4, VCM/UTM are acceptable. Swab tips may be polyester, rayon, nylon flocked. Flexible shafts must be used for proper collection. Accuracy of the test depends on the collection of ciliated epithelial cells material. Half the distance from the nostril to the base of the ear could be used as estimation of the site of collection. Gently insert swab into the nostril. Keep the swab near the septum floor of the nose while gently pushing into the posterior nasopharynx. Rotate swab 2-3 times and hold in place for a few seconds for optimal collection. Collection of both nostrils is recommended but not required. |
| Transport container | Nasopharygeal aspirate in a sterile vial or nasopharyngeal swab in Amies liquid (red-cap) medium |
| Transport temperature | Refrigerated (cold packs) |
| Specimen stability | Nasopharyngeal aspirate Room temperature: 48 hours Refrigerated: 8 days Frozen: 30 days Nasopharyngeal swab Room temperature: 7 days Refrigerated: 7 days Frozen: 30 days |
| Reject criteria | Collection tubes with Amies gel or charcoal containing media • Samples collected using cotton or calcium alginate tipped swabs • Rigid shaft swabs |
Reference Range(s)
| B. pertussis DNA | Not detected |
| B. parapertussis DNA | Not detected |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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