Neisseria gonorrhoeae RNA, TMA, Urogenital
Test code
CPT code(s)*
Test resources
Specimen handling - specimen collection guide
Details
Methodology
Transcription-Mediated Amplification (TMA)
Alternative name(s)
NG TMA,GC Aptima,NG Aptima,Hologic,GC TMA
Specimen requirements | |
|---|---|
| Patient preparation | Urine: Patient should not have urinated within one hour prior to collection. Female patients should not cleanse the labial area prior to providing the specimen. |
| Preferred specimen(s) | 1 vaginal swab in Aptima® Transport Tube or 1 endocervical or urethral swab in Aptima® Transport Tube or 2 mL urine in Aptima® Transport Tube or 1 mL liquid cytology ThinPrep® PreservCyt® in Aptima® Transfer Tube or 0.5 mL SurePath™ Preservative Fluid in Aptima® Transfer Tube |
| Minimum volume | 1 vaginal swab in Aptima® Transport • 1 endocervical or urethral swab in Aptima® Transport • 2 mL urine in Aptima® Transport • 1 mL ThinPrep® PreservCyt® in Aptima® Transfer Tube • 0.5 mL SurePath™ fluid in Aptima® Transfer Tube |
| Collection instructions | Vaginal Swab: Follow instructions in the Aptima® Multitest Collection Kit (orange label). Endocervical or Urethral Swab: Follow instructions in the Aptima® Unisex Swab Specimen Collection Kit (white label). In females, to ensure collection of cells infected with N. gonorrhoeae, columnar epithelial cells lining the endocervix should be obtained. Excess mucus should be removed prior to sampling. Urine: Direct patient to provide a first-catch urine (a maximum of 20-30 mL of the initial urine stream) into a urine collection cup free of any preservatives. 2 mL of urine specimen must be transferred into the Aptima® Specimen Transport Tube within 24 hours of collection and before being assayed. Use the tube provided in the urine specimen collection kit. The fluid (urine plus transport media) level in the urine transport tube must fall within the clear pane of the tube label. Labs performing cytology: Aliquot ThinPrep® PreservCyt® solution before performance of liquid-based cytology testing. ThinPrep® PreservCyt®: Transfer 1 mL ThinPrep® PreservCyt® solution into Aptima® Specimen Transfer Tube (green label) or Aptima® Multitest Collection Tube (orange label) or Unisex Swab Specimen Collection Kit (white label). SurePath™: SurePath™ fluid must be transferred to Aptima® tube within 4 days of collection. Transfer 0.5 mL SurePath™ solution into Aptima® Specimen Transfer Tube (green label) or Aptima® Multitest Collection Tube (orange label) or Unisex Swab Specimen Collection Kit (white label). For a complete list of assay limitations we recommend that you review the manufacturers package insert, https://www.hologic.com/package-inserts/diagnostic-products/aptima-combo-2-assay-ctng |
| Transport container | Aptima® Transport Tube |
| Transport temperature | Room temperature |
| Specimen stability | Swabs in Aptima® Transport Tube Liquid cytology ThinPrep® Preservcyt® |
| Reject criteria | Transport tubes with 2 swabs • Transport tubes with Non-Aptima® swabs • Swab transport tubes with no swab • Specimens in broken containers • Swab submitted in viral transport media • Urine samples where the fluid level is not between the black fill lines • ThinPrep® PreservCyt® material previously processed for cytology • ThinPrep® PreservCyt® with excess mucus |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
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