Cortisol, Total, LC/MS

Test Code

11281

CPT Code(s)

82533

Test Code

11281

CPT Code(s)

82533

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Cortisol, Total, LC/MS - Total cortisol concentrations are decreased in Addison's Disease and increased in Cushing's Disease and in other conditions of glucocorticoid excess.

Test Resources

None found for this test
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Test Details

Methodology

Chromatography/Mass Spectrometry

Assay Category

This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.

Reference Range(s)

Adult
8:00 - 10:00 AM	4.6-20.6 mcg/dL
4:00 - 6:00 PM	1.8-13.6 mcg/dL

Cortisol Response to ACTH
Peak	>20.0 mcg/dL
Peak	>16.0 mcg/dL after IM injection

Pediatric

Age	mcg/dL
Infants
Premature (31-35 weeks)**	≤15.0
Term, 3 days old**	≤14.0
<30 days	Not established
1-11 months	1.4-24.0
1 year	1.7-25.4
2 years	1.8-26.4
3 years	2.0-27.0
4 years	2.0-27.2
5 years	2.0-27.3
6 years	2.0-27.2
7 years	2.0-27.1
8 years	2.0-27.0
9 years	1.9-26.8
10 years	1.9-26.4
11 years	1.8-26.1
12 years	1.8-26.0
13 years	1.8-26.1
14 years	1.9-26.6
15 years	2.1-27.5
16 years	2.3-28.6
17 years	2.6-29.8

Pediatric lower reference values are reflective of both AM and early PM sampling
⁠⁠⁠⁠⁠⁠⁠

ACTH Stimulation**	Baseline (mcg/dL)	60 Minutes (mcg/dL)
1-12 Months	3.0-23.0	32.0-60.0
1-5 Years	6.0-25.0	22.0-40.0
6-12 Years	3.0-15.0	17.0-28.0

**Pediatric data from J Clin Endocrinol Metab
(1991) 73:674-686 and J Clin Endocrinol Metab
(1989) 69:1133-1136.

LOINC® Codes, Performing Laboratory

Service Area must be determined

Methodology

Chromatography/Mass Spectrometry

Assay Category

Reference Range(s)

Adult
8:00 - 10:00 AM	4.6-20.6 mcg/dL
4:00 - 6:00 PM	1.8-13.6 mcg/dL

Cortisol Response to ACTH
Peak	>20.0 mcg/dL
Peak	>16.0 mcg/dL after IM injection

Pediatric

Age	mcg/dL
Infants
Premature (31-35 weeks)**	≤15.0
Term, 3 days old**	≤14.0
<30 days	Not established
1-11 months	1.4-24.0
1 year	1.7-25.4
2 years	1.8-26.4
3 years	2.0-27.0
4 years	2.0-27.2
5 years	2.0-27.3
6 years	2.0-27.2
7 years	2.0-27.1
8 years	2.0-27.0
9 years	1.9-26.8
10 years	1.9-26.4
11 years	1.8-26.1
12 years	1.8-26.0
13 years	1.8-26.1
14 years	1.9-26.6
15 years	2.1-27.5
16 years	2.3-28.6
17 years	2.6-29.8

Pediatric lower reference values are reflective of both AM and early PM sampling
⁠⁠⁠⁠⁠⁠⁠

ACTH Stimulation**	Baseline (mcg/dL)	60 Minutes (mcg/dL)
1-12 Months	3.0-23.0	32.0-60.0
1-5 Years	6.0-25.0	22.0-40.0
6-12 Years	3.0-15.0	17.0-28.0

**Pediatric data from J Clin Endocrinol Metab
(1991) 73:674-686 and J Clin Endocrinol Metab
(1989) 69:1133-1136.

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

1 mL serum collected in a red-top tube (no gel)

Alternative Specimen(s)

Plasma collected in: EDTA (lavender-top) tube

Minimum Volume

0.2 mL

Collection Instructions

Specify time of day specimen was collected

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year

Reject Criteria

Hemolysis • Lipemia • Serum separator tube (SST)

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

1 mL serum collected in a red-top tube (no gel)

Alternative Specimen(s)

Plasma collected in: EDTA (lavender-top) tube

Minimum Volume

0.2 mL

Collection Instructions

Specify time of day specimen was collected

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 14 days
Refrigerated: 21 days
Frozen: 1 year

Reject Criteria

Hemolysis • Lipemia • Serum separator tube (SST)

Setup Schedule

Service Area must be determined

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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