Glucose Tolerance Test, 6 specimens
Test Code
Clinical Significance
Glucose Tolerance Test, 6 specimens - This test is used for the routine diagnosis of diabetes in children and the non- pregnant adult. For pregnant females use test code 6745. For appropriate interpretation of this test, the patient must fast overnight and ingest a 75 g load of glucose, immediately after a fasting specimen is obtained. (for children, the glucose load is 1.75 g/kg of body weight, up to 75). The diagnosis of diabetes is made if glucose exceeds 200 mg/dL in the 2 hour specimen and in any preceding specimen.
Test Resources
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Test Details
Fasting required. Fasting is defined as no consumption of food or beverage other than water for at least 8 hours before testing.
Methodology
Reference Range(s)
|
American Diabetes Association |
|||||||
| Glucose Value (mg/dL) | |||||||
| Interpretation | Fasting | 1 Hr Tolerance | 2 Hr Tolerance | ||||
| Normal | <100 | Not established | <140 | ||||
| Impaired Fasting | 100-125 | ||||||
| Impaired Tolerance | 140-199 | ||||||
| Diabetes | ≥126* | ≥200* | |||||
| *Must be confirmed by testing on a subsequent day. | |||||||
Specimen 1: 65-99 mg/dL
Preferred Specimen(s)
Six - 1 mL plasma specimens collected in fluoride/oxalate (gray-top) tubes
Alternative Specimen(s)
Plasma from fluoride/oxalate (gray-top) tube in a plastic screw-cap vial • Serum (separated from cells immediately) in a plastic screw-cap vial • Spun SST • Plasma from lithium heparin (green-top) tube in a plastic screw-cap vial • Plasma from sodium heparin (green-top) tube in a plastic screw-cap vial
Minimum Volume
0.5 mL for each specimen
Collection Instructions
Immediately after a fasting specimen is obtained, have patient ingest a 75 g load of glucose. Draw subsequent specimens per physician's order. If not specified by the physician, draw specimens 1, 2, 3, 4 and 5 hours later. Label each with the specific draw time. Serum and plasma submissions must be separated from cells.
Transport Container
Fluoride/oxalate (gray-top) tubes
Transport Temperature
Room temperature
Specimen Stability
Serum:
- Room temperature: 4 days
- Refrigerated: 7 days
- Frozen: 28 days
- Whole blood (gray-top) tube:
- Room temperature: 24 hours
- Refrigerated: 72 hours
- Frozen: Unacceptable
- Sodium fluoride plasma:
- Room temperature: 7 days
- Refrigerated: 7 days
- Frozen: 28 days
- Heparinized plasma:
- Room temperature: 48 hours
- Refrigerated: 7 days
- Frozen: 28 days
Reject Criteria
Unspun SST • Red-top or non-plasma separator (PST) green-top tubes • Anticoagulants other than lithium heparin, sodium heparin or fluoride/oxalate
Setup Schedule
Six - 1 mL plasma specimens collected in fluoride/oxalate (gray-top) tubes
Immediately after a fasting specimen is obtained, have patient ingest a 75 g load of glucose. Draw subsequent specimens per physician's order. If not specified by the physician, draw specimens 1, 2, 3, 4 and 5 hours later. Label each with the specific draw time. Serum and plasma submissions must be separated from cells.
Serum:
Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days
Whole blood (gray-top) tube:
Room temperature: 24 hours
Refrigerated: 72 hours
Frozen: Unacceptable
Sodium fluoride plasma:
Room temperature: 7 days
Refrigerated: 7 days
Frozen: 28 days
Heparinized plasma:
Room temperature: 48 hours
Refrigerated: 7 days
Frozen: 28 days