Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum

Test Code

10269

CPT Code(s)

84155, 84165

Test Code

10269

CPT Code(s)

84155, 84165

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Clinical Significance

Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum - Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).

Test Resources

Clinical Focus

Parathyroid Dysfunction: Laboratory Testing for Diagnosis and Management

Test Details

Includes

If abnormal banding is detected, then Immunofixation will be performed at an additional charge (CPT code(s): 86334).

Patient Preparation

Overnight fasting is preferred

Methodology

Capillary Zone Electrophoresis • Spectrophotometry (SP)

Reference Range(s)

Protein, Total, Serum
	Male (g/dL)	Female (g/dL)
<1 Month	4.1-6.3	4.2-6.2
1-5 Months	4.7-6.7	4.4-6.6
6-11 Months	5.5-7.0	5.6-7.9
1-19 Years	6.3-8.2	6.3-8.2
≥20 Years	6.1-8.1	6.1-8.1

Protein Electrophoresis
Albumin	3.8-4.8 g/dL
Alpha-1 Globulin	0.2-0.3 g/dL
Alpha-2 Globulin	0.5-0.9 g/dL
Beta-1 Globulin	0.4-0.6 g/dL
Beta-2 Globulin	0.2-0.5 g/dL
Gamma Globulin	0.8-1.7 g/dL
Abnormal Protein Band 1	None detected
Abnormal Protein Band 2	None detected
Abnormal Protein Band 3	None detected

Alternative Name(s)

Electrophoresis, IFE,SPE with Reflex IFE,SPEP

LOINC® Codes, Performing Laboratory

Service Area must be determined

Includes

If abnormal banding is detected, then Immunofixation will be performed at an additional charge (CPT code(s): 86334).

Patient Preparation

Overnight fasting is preferred

Methodology

Capillary Zone Electrophoresis • Spectrophotometry (SP)

Reference Range(s)

Protein, Total, Serum
	Male (g/dL)	Female (g/dL)
<1 Month	4.1-6.3	4.2-6.2
1-5 Months	4.7-6.7	4.4-6.6
6-11 Months	5.5-7.0	5.6-7.9
1-19 Years	6.3-8.2	6.3-8.2
≥20 Years	6.1-8.1	6.1-8.1

Protein Electrophoresis
Albumin	3.8-4.8 g/dL
Alpha-1 Globulin	0.2-0.3 g/dL
Alpha-2 Globulin	0.5-0.9 g/dL
Beta-1 Globulin	0.4-0.6 g/dL
Beta-2 Globulin	0.2-0.5 g/dL
Gamma Globulin	0.8-1.7 g/dL
Abnormal Protein Band 1	None detected
Abnormal Protein Band 2	None detected
Abnormal Protein Band 3	None detected

Alternative Name(s)

Electrophoresis, IFE,SPE with Reflex IFE,SPEP

LOINC® Codes, Performing Laboratory

Service Area must be determined

Preferred Specimen(s)

4 mL serum

Minimum Volume

2 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Grossly icteric • Plasma

Setup Schedule

Service Area must be determined

Preferred Specimen(s)

4 mL serum

Minimum Volume

2 mL

Transport Container

Transport tube

Transport Temperature

Room temperature

Specimen Stability

Room temperature: 4 days
Refrigerated: 7 days
Frozen: 28 days

Reject Criteria

Gross hemolysis • Grossly lipemic • Grossly icteric • Plasma

Setup Schedule

Service Area must be determined

Not offered in Quest Diagnostics Nichols Institute (IFD) – San Juan Capistrano. Please provide SERVICE AREA INFORMATION to find available tests you can order.

In-home collection may be available in your area for purchase through Quest Mobile.

Test Details

Clinical Significance

Test Resources

Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.

The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.

This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.

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