Micronutrients, B-Vitamin Panel
Test code
CPT code(s)*
Test resources
Please visit our Clinical Education Center to stay informed on any future publications, webinars, or other education opportunities.
Details
Includes
10188 - Micronutrient, Folate
10189 - Micronutrient, Vitamin B1 (Thiamine)
10190 - Micronutrient, Vitamin B2
10191 - Micronutrient, Vitamin B3
10192 - Micronutrient, Vitamin B5
10193 - Micronutrient, Vitamin B6
10194 - Micronutrient, Vitamin B12
Methodology
Immunoassay (IA) • Liquid Chromatography/Tandem Mass Spectrometry (LC/MS/MS)
Assay category
This test was developed and its analytical performance characteristics have been determined by Quest Diagnostics. It has not been cleared or approved by FDA. This assay has been validated pursuant to the CLIA regulations and is used for clinical purposes.
Specimen requirements | |
|---|---|
| Patient preparation | Overnight fasting is required. Avoid vitamin supplements for 24 hours prior to collection. |
| Preferred specimen(s) | 4 mL serum collected in a red-top tube (no gel) - protected from light and 3 mL frozen plasma collected in an EDTA (lavender-top) tube - protected from light and 2 mL frozen whole blood collected in an EDTA (lavender-top) tube - protected from light |
| Minimum volume | 2 mL serum • 1 mL EDTA plasma • 1 mL EDTA whole blood |
| Collection instructions | Patients should be 18 years of age or greater. Folate & Vitamin B12: Transfer 2 mL (1 mL min) serum into plastic transport tube, ship frozen. Vitamin B2, B3, B5: Transfer 2 mL (1 mL min) serum into an amber vial (or wrap with aluminum foil), ship frozen. Vitamin B6: Transfer 3 mL (1 mL min) EDTA plasma into an amber vial (or wrap with aluminum foil), ship frozen. Separate cells within 24 hours by centrifugation for 5-10 minutes. Transfer plasma to an amber polypropylene or polyethylene transport tube to protect from light. Alternately, neutral color polypropylene or polyethylene tube can be used if wrapped in aluminum foil. Freeze the tubes at -10 to -30° C. Ship frozen. Vitamin B1: Transfer 3 mL (1 mL min) EDTA whole blood into an amber vial (or wrap with aluminum foil), ship frozen. Dietary supplements containing biotin may interfere in assays and may skew analyte results to be either falsely high or falsely low. For patients receiving the recommended daily doses of biotin, draw samples at least 8 hours following the last biotin supplementation. For patients on mega-doses of biotin supplements, draw samples at least 72 hours following the last biotin supplementation. Remove serum or plasma from cells within 4 hours of collection. |
| Transport container | Amber polypropylene or polyethylene tubes or Neutral color polypropylene or Polyethylene tubes wrapped in aluminum foil to protect from light |
| Transport temperature | Frozen |
| Specimen stability | Folate and Vitamin B12 Serum Room temperature: 36 hours Refrigerated: 7 days Frozen: 28 days Vitamin B2, B3 and B5 Serum Room temperature: Unacceptable Refrigerated: Unacceptable Frozen: 30 days Vitamin B6 Plasma Room temperature: 6 hours Refrigerated: 12 hours Frozen: 6 days Vitamin B1 Whole Blood Room temperature: 72 hours Refrigerated: 5 days Frozen: 35 days |
| Reject criteria | Hemolysis • Lipemia • Received room temperature • Received refrigerated • Not protected from light • Serum separator tube (SST) • Plasma collected in gel tube |
Reference Range(s)
≥18 years
| Folate | >5.4 ng/mL |
| Vitamin B1 (Thiamine) | 78-185 nmol/L |
| Vitamin B2 | 6.2-39.0 nmol/L |
| Vitamin B3 | ≤110 ng/mL |
| Vitamin B5 | <275 ng/mL |
| Vitamin B6 | 5.1-54.8 ng/mL |
| Vitamin B12 | 200-1100 ng/mL |
Reference ranges are provided as general guidance only. To interpret test results use the reference range in the laboratory report.
*The CPT codes provided are based on AMA guidance and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.
This material contains content from LOINC® (http://loinc.org). The LOINC Table, LOINC Table Core are copyright © 1995-2019, Regenstrief Institute, Inc. and the Logical Observation Identifiers Names and Codes (LOINC) Committee and is available at no cost under the license at http://loinc.org/license.
The tests listed by specialty and category are a select group of tests offered. For a complete list of Quest Diagnostics tests, please adjust the filter options chosen, or refer to our Directory of Services.